Rat Toll-like receptor 9,TLR-9 ELISA kit

Instructions
Code CSB-E09864r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Toll-like receptor 9,TLR-9
Abbreviation TLR9/CD289
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.31 ng/mL-20 ng/mL
Sensitivity 0.08 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TLR-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 107    
Range % 100-111    
1:2 Average % 104    
Range % 97-107    
1:4 Average % 98    
Range % 89-103    
1:8 Average % 104    
Range % 97-108    
Recovery
The recovery of rat TLR-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 98 91-103    
EDTA plasma (n=4) 103 97-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.132 1.999 2.066 1.964    
10 1.292 1.268 1.280 1.178    
5 0.814 0.795 0.805 0.703    
2.5 0.464 0.452 0.458 0.356    
1.25 0.273 0.291 0.282 0.180    
0.62 0.204 0.227 0.216 0.114    
0.31 0.148 0.155 0.152 0.050    
0 0.104 0.100 0.102      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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