Rat Transferrin receptor protein 1/CD antigen CD71 (TFR/CD71) ELISA Kit

Instructions
Code CSB-E08388r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Transferrin receptor protein 1/CD antigen CD71 (TFR/CD71)
Alternative Names Tfrc ELISA Kit; Trfr ELISA Kit; Transferrin receptor protein 1 ELISA Kit; TR ELISA Kit; TfR ELISA Kit; TfR1 ELISA Kit; Trfr ELISA Kit; CD antigen CD71 ELISA Kit; Fragment ELISA Kit
Abbreviation TFR/CD71
Uniprot No. Q99376
Species Rattus norvegicus (Rat)
Sample Types serum, plasma,homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TFR/CD71 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 98    
Range % 88-102    
1:2 Average % 103    
Range % 93-108    
1:4 Average % 90    
Range % 83-94    
1:8 Average % 95    
Range % 87-99    
Recovery
The recovery of rat TFR/CD71 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 93 86-97    
EDTA plasma (n=4) 103 93-107    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
2000 2.081 2.132 2.107 2.005    
1000 1.441 1.411 1.426 1.324    
500 0.958 0.927 0.943 0.841    
250 0.674 0.646 0.660 0.558    
125 0.360 0.345 0.353 0.251    
62.5 0.254 0.247 0.251 0.149    
31.25 0.176 0.171 0.174 0.072    
0 0.101 0.103 0.102      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Positively regulates T and B cell proliferation through iron uptake (By similarity).
Subcellular Location Cell membrane, Single-pass type II membrane protein, Melanosome
Protein Families Peptidase M28 family, M28B subfamily
Tissue Specificity In testis, expressed in Sertoli cells, peritubular myoid cells and in germinal cells. Highest levels in Sertoli cells.
Database Links

STRING: 10116.ENSRNOP00000002407

UniGene: Rn.98672

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1