Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit

Instructions
Code CSB-EL024012RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name troponin I type 2 (skeletal, fast)
Alternative Names Tnni2 ELISA Kit; Trp1 ELISA Kit; Troponin I ELISA Kit; fast skeletal muscle ELISA Kit; Troponin I ELISA Kit; fast-twitch isoform ELISA Kit
Abbreviation TNNI2
Uniprot No. P27768
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 106
Range % 103-108
1:2 Average % 96
Range % 91-100
1:4 Average % 92
Range % 87-95
1:8 Average % 95
Range % 89-98
Recovery
The recovery of rat TNNI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 96-108
EDTA plasma (n=4) 94 87-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.841 2.844 2.843 2.712
400 2.142 2.214 2.178 2.047
200 1.433 1.474 1.454 1.323
100 0.870 0.890 0.880 0.749
50 0.569 0.577 0.573 0.442
25 0.453 0.474 0.464 0.333
12.5 0.255 0.236 0.246 0.115
0 0.135 0.126 0.131  
.
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. The effects of reactive oxygen species on tryptophan residues in alpha skeletal actin and troponin I, was investigated. PMID: 20455213
  2. Release of fast sTnI consistent with load-induced injury of fast glycolytic fibres of inspiratory muscles, probably diaphragm. May provide insights into molecular basis of respiratory muscle dysfunction. Marker of respiratory muscle fatigue. PMID: 14673191
Protein Families Troponin I family
Database Links

KEGG: rno:29389

STRING: 10116.ENSRNOP00000027487

UniGene: Rn.9924

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