Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit

Instructions
Code CSB-EL024015RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name troponin T type 1 (skeletal, slow)
Alternative Names Tnnt1 ELISA Kit; Fang2 ELISA Kit; Troponin T ELISA Kit; slow skeletal muscle ELISA Kit; TnTs ELISA Kit; Slow skeletal muscle troponin T ELISA Kit; sTnT ELISA Kit
Abbreviation TNNT1
Uniprot No. Q7TNB2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 0.97 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-96
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat TNNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
250 2.245 2.371 2.308 2.177
125 1.744 1.815 1.780 1.649
62.5 1.141 1.172 1.157 1.026
31.25 0.713 0.701 0.707 0.576
15.6 0.424 0.413 0.419 0.288
7.8 0.274 0.288 0.281 0.150
3.9 0.187 0.194 0.191 0.060
0 0.130 0.131 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Families Troponin T family
Tissue Specificity Expressed in soleus muscle. Isoform 4 is predominantly expressed in fast muscles.
Database Links

KEGG: rno:171409

STRING: 10116.ENSRNOP00000038327

UniGene: Rn.13846

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