Home
Kit
ELISA Kit
Rat Tumor necrosis factor β,TNF-β ELISA KIT

Rat Tumor necrosis factor β,TNF-β ELISA KIT

Citations (3) Protocol

Code	CSB-E07379r
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations (3)
Customer Reviews and Q&A
Target Background

Product Details

Target Name

Tumor necrosis factor β,TNF-β

Alternative Names

Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit

Abbreviation

TNF-β

Uniprot No.

Q06332

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	96-105
1:2	Average %	102
	Range %	97-107
1:4	Average %	87
	Range %	83-94
1:8	Average %	96
	Range %	91-102

Recovery

The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99108
EDTA plasma (n=4)	88	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.632	2.765	2.699	2.614
250	2.212	2.302	2.257	2.172
125	1.545	1.577	1.561	1.476
62.5	0.765	0.771	0.768	0.683
31.2	0.411	0.423	0.417	0.332
15.6	0.257	0.264	0.261	0.176
7.8	0.176	0.185	0.181	0.096
0	0.084	0.085	0.085

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Protective effects of aucubin against nonylphenol-induced liver toxicity by improving biochemical, inflammatory and histopathological indices A Rehman,Journal of King Saud University - Science,2022
Hepatoprotective role of vitexin against cadmium-induced liver damage in male rats: A biochemical, inflammatory, apoptotic and histopathological … KK Noor,Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,2022
The Cu-containing TiO2 coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium substrates QianliHuang.et al,Colloids and Surfaces B: Biointerfaces,2018

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function (From Uniprot)	Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
Gene References into Functions	Adrenomedullin can increase the expression of TNF-beta and TNFR in renal early trauma. PMID: 20092780 TNFbeta-so far only known to be produced by lymphocytes-was demonstrated in alveolar macrophages, bronchial epithelial cells, vascular smooth muscle cells and endothelial cells at the protein and the message level PMID: 12849708
Subcellular Location	Secreted. Membrane.
Protein Families	Tumor necrosis factor family
Database Links	KEGG: rno:25008 STRING: 10116.ENSRNOP00000001111 UniGene: Rn.160577

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TNFR1: A Multifaceted Molecule in Cancer, Another Promising Therapeutic Target for COVID-19 Surface Markers That Help You Identify Lymphocytes How do Th1 and Th2 Cells Differentiate? Does TNF Superfamily Inhibit Cancer or Promote Its Development? ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

NF-kappa B signaling pathway TNF signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St. Ste 610-312, Houston, TX 77054, USA

Join Us with

Subscribe newsletter

Leave a message

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：