Product Details

Target Name

UDP glucuronosyltransferase 1 family, polypeptide A1

Alternative Names

Ugt1a1; Ugt1; UDP-glucuronosyltransferase 1A1; UGT1A1; B1; UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1

Abbreviation

UGT1A1

Uniprot No.

Q64550

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.2 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat UGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	110
	Range %	104-114

Recovery

The recovery of rat UGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.941	2.014	1.978	1.875
1000	1.503	1.498	1.501	1.398
500	0.999	1.031	1.015	0.912
250	0.610	0.631	0.621	0.518
125	0.417	0.407	0.412	0.309
62.5	0.235	0.228	0.232	0.129
31.25	0.148	0.150	0.149	0.046
0	0.101	0.104	0.103

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

The Rat UDP-glucuronosyltransferase 1(UGT1) ELISA Kit is designed for reliable and accurate quantification of UGT1 levels in rat serum, plasma, and tissue homogenates.

The UGT1 enzyme, also known as UDP-glucuronosyltransferase 1A1 (UGT1A1) is a key player in the metabolism of drugs and toxins in the liver, making it an essential target for research in the field of metabolism. This kit has a wide detection range of 31.2 pg/mL to 2000 pg/mL with a sensitivity of 7.8 pg/mL, allowing for precise measurement of UGT1 levels in various samples.

With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is efficient and convenient for high-throughput screening. The detection wavelength is 450 nm and the assay principle is quantitative, utilizing the sandwich method for accurate and specific detection of UGT1.

This ELISA kit is ideal for researchers working in the field of metabolism and drug development. Trust in our reliable and validated Rat UGT1 ELISA Kit to facilitate your research and drive new discoveries.

Target Background

Function
(From Uniprot)

UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol. Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates. Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties. Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II. Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan.

Gene References into Functions

study indicated that Status Epilepticus altered the expression of brain Ugt1a1 and Ugt1a7, which could alter glucuronidation in the brain. PMID: 29131354
UGT1A1 controls estrogen metabolism. PMID: 28479355
Data indicate that the up-regulation of phase II detoxification in the DR group was confirmed by mRNA and protein expression levels of uridinediphospho-glucuronosyltransferase and glycine-N-acyltransferase in actual liver tissues. PMID: 23230277
Both fipronil and fipronil sulfone treatments induced a 2.5-fold increase in Ugt1a1 and Sult1b1 messenger RNA (mRNA) expressions. PMID: 22447239
UDP-glucuronosyltransferase (UDP-GT) activities towards p-nitrophenol were markedly increased in rats that consumed tea.Induction of UDP-glucuronosyltransferase activity by tea may involve the UDP-GT1 (UGT1A) gene complex of the UDP-GT multigene family PMID: 11983278
These results indicate that the expression of extrahepatic UDP-glucuronosyltransferases UGT1a1 and UGT1a6 is isoform-specific and regulated differentially in tissues by the pituitary gland. PMID: 12153725
Our findings show that the absence of UGT1 protein from Gunn rat hepatocytes is due to rapid degradation of the truncated UGT1 protein by the proteasome and elucidate the molecular basis underlying the deficiency in bilirubin glucuronidation. PMID: 12220528
Developmental stage-specific switching of regulation of the rat UGT1 gene complex was found. PMID: 18938141
Data suggest that thyroid hormones and vitamin A are co-regulators of UDP-glucuronosyltransferase (UGT) 1 expression, without affecting the UGT2 family PMID: 11997190

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

UDP-glycosyltransferase family

Database Links

KEGG: rno:24861

STRING: 10116.ENSRNOP00000025045

UniGene: Rn.26489

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Code	CSB-E13845r
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