Rat angiotensin Ⅱ receptor type 1(AT1R)ELISA Kit

Instructions
Code CSB-E13746r
Size 96T,5×96T,10×96T
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Product Details

Target Name angiotensin II receptor, type 1
Alternative Names Agtr1 ELISA kit; Agtr1a ELISA kit; At1aType-1A angiotensin II receptor ELISA kit; Angiotensin II type-1A receptor ELISA kit; AT1A ELISA kit
Abbreviation AGTR1
Uniprot No. P25095
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AT1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %87-97
1:2Average %97
Range %95-102
1:4Average %101
Range %95-105
1:8Average %94
Range %91-98
Recovery
The recovery of rat AT1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9995-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.931 2.874 2.903 2.757
25002.398 2.455 2.427 2.281
12501.799 1.740 1.770 1.624
6250.923 0.984 0.954 0.808
3120.545 0.589 0.567 0.421
1560.477 0.486 0.482 0.336
780.280 0.259 0.270 0.124
00.141 0.151 0.146
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Gene References into Functions
  1. Following restraint stress, AT1 receptors in the pre-limbic cortex modulate the acute restraint stress-evoked pressor response. PMID: 28695320
  2. A possible reduction in CB1R functionality, coupled with a hyperfunctional AT1R in the brainstem, could well be significant factors in the development of hypertensive states. AT1R-mediated phosphorylation of CB1R could be critical for impaired cerebellar development characterized by a hyperactive RAS PMID: 28887229
  3. In the aged offspring of prenatal high sucrose exposure rats, mRNA and protein expressions of AT1R gene in both large and small blood vessels were significantly increased. PMID: 27818235
  4. AT1R activation results in translocation of RGS5 toward the plasma membrane, limiting AT1R-mediated vasoconstriction through its role in Gq/11 protein-dependent signaling. PMID: 29061726
  5. The present studies support growing evidence for novel modes of activation of the AT1 R in arterioles and suggest that mechanically activated AT1 R generates diacylglycerol, which in turn activates PKC which induces the actin cytoskeleton reorganization that is required for pressure-induced vasoconstriction PMID: 27531064
  6. Altered expression of AT1 and AT2 receptors with aging may induce mitochondrial dysfunction, the main risk factor for neurodegeneration. PMID: 27763643
  7. In this study, we demonstrate that vitamin D improved many factors associated with PE and reduced blood pressure in a hypertensive model without PE features, indicating that vitamin D could be beneficial for various hypertensive disorders of pregnancy. PMID: 27903510
  8. Results demonstrated that uric acid can stimulate p38 MAPK, p44/42 MAPK and PDGFRbeta phosphorylation in a time- and concentration-dependent manner. Furthermore, treatment of VSMCs with the angiotensin II type I receptor (AT1R) inhibitor losartan suppressed p38 MAPK and p44/42 MAPK induction by uric acid. PMID: 27400779
  9. the AT1 expression in neuronal subpopulation of lower lumbar dorsal root ganglia under normal conditions and its alteration in neuropathic pain model, is reported. PMID: 27319015
  10. results suggest that the AT1 receptor is involved in development of hypertension and cardiac fibrosis PMID: 27085217
  11. Data indicate the effect of angiotensin II receptors' blockade on the macrophage infiltration and histone modifications occurring at the promoter region of monocyte chemoattractant protein (MCP-1) and transforming growth factor-beta (TGF-beta1). PMID: 27693081
  12. Vinegar activates AMPK by increasing AMP/ATP ratios, thereby increases PGC-1alpha and PPARgamma expressions, and inhibits AT1R expression in spontaneously hypertensive rats. PMID: 26476634
  13. AT1R induces the redox-sensitive signaling pathway in the brain stem. PMID: 26575857
  14. The prothrombotic effect of aldosterone is partially mediated by the AT1 receptor. PMID: 26709040
  15. aldosterone induced an inflammatory response in rat mesangial cells cultured in high-glucose media via the AT1a and AT2 pathways. PMID: 24464860
  16. in diabetes, AT1R increases prostaglandins E2 (PGE2) generation and reduces conversion of PGE2 to PGF2alpha with the progression of diabetes. PMID: 26195268
  17. Previously unappreciated functions of AT1aR helix 8 and novel mechanisms governing the cell surface transport and function of AT1aR. PMID: 26342563
  18. Results support functional interactions between AT1 receptors and TLR4 in mediating ANG II-dependent microglial activation and oxidative stress within the hypothalamic paraventricular nucleus. PMID: 26637556
  19. Endogenous hydrogen sulfide reduced the liver expression levels of AGTR1, which may be associated with the delayed progression of hepatic fibrosis. PMID: 26034979
  20. The role of angiotensin II type I receptor (AT1-AA) in hypertension and preeclampsia in rats is reported. PMID: 26310940
  21. These findings indicate that biased signaling of the beta-arrestin pathway through the AT1 receptor inhibits NHE3 activity in the proximal tubule at least in part due to changes in NHE3 subcellular localization. PMID: 26246427
  22. AVP-induced increase in AQP2 is blunted in heart failure during cardiac remodeling and is associated with decreased AT1R abundance in rat kidney. PMID: 25658446
  23. in rats with post-infarction heart failure the regulation of blood pressure by AT1Rs depends on centrally acting endogenous TNF-alpha PMID: 25481865
  24. Heightened AT1aR expression in spontaneously hypertensive rats is related to the AT1aR promoter hypo-methylation. PMID: 25596947
  25. Clathrin-dependent internalization of the angiotensin II ATA receptor links receptor internalization to COX-2 protein expression in rat aortic vascular smooth muscle cells PMID: 25542758
  26. our results point out multiple actions of Ang II in glioma pathogenesis and reveal the participation of both Ang II receptors in the regulation of genes relevant for glioma progression. PMID: 25365520
  27. Vascular oxidative can upregulate AT1R, via mechanisms involving NFkappaB, and contribute to the development of hypertension. PMID: 25198883
  28. These findings suggest that an interaction between AT1-R and IL-1beta in the paraventricular nucleus contributes to progression in heart failure. PMID: 24955935
  29. We conclude that Ang II pretreatment, as a trigger, activated the AT1R/HIF-1alpha/ACE axis that then mediated Ang II-induced VEGF synthesis in MSCs. PMID: 25401104
  30. Venthamarai chooranam, a polyherbal Siddha medicine, alleviates hypertension via ATR and eNOS signaling pathway in 2K1C hypertensive rats PMID: 24719376
  31. Hyperhomocysteinemia accelerates collagen accumulation in the adventitia of balloon-injured rat carotid arteries via angiotensin II type 1 receptor. PMID: 25350112
  32. The Ang II AT1 receptors in Central nucleus of the amygdala are involved in the anxiety state induced by stress in the fear-potentiated plus-maze behaviour. PMID: 25003108
  33. Western blot analysis revealed that AT1 expression increased in the control group during aging while AT2 and Mas remained unchanged PMID: 24819472
  34. Arrestins coordinate AT1 receptor regulation of ERK1/2 and Akt activity and stimulate protein translation via both Akt-mTOR-p70/85S6K and ERK1/2-p90RSK pathways. PMID: 25081544
  35. A counterregulatory interaction between dopamine and the angiotensin receptor was observed. PMID: 24529758
  36. Serum levels of ghrelin are significantly positively correlated with Ang II in chronic heart failure (CHF) patients and that ghrelin can inhibit Ang II-induced cardiomyocyte apoptosis by down-regulating AT1R, thereby playing a role in preventing HF. PMID: 24465706
  37. angiotensin signaling through Agtr1a in the subfornical organ contributes to increased water intake, but not dilutional hyponatremia, during congestive liver failure. PMID: 25009217
  38. The enhanced downregulation of the AT1-B receptors in the renin-negative SMCs in the efferent arterioles demonstrates that the regulation of the glomerular filtration rate by the pre- and postglomerular arterioles is changed in diabetes PMID: 24587998
  39. 2-methoxyestradiol down-regulation of angiotensin AT1 receptor is dependent on endoplasmic reticulum membrane-associated GRP30. PMID: 24262995
  40. Interaction between AT1 receptor and nuclear factor (NF)-kappaB in the paraventricular nucleus contributes to oxidative stress in heart failure patients. PMID: 23877628
  41. Our results are the first to show that activation of the brain RAS after surgery contributes to POCD in aged rats. PMID: 24814703
  42. Data suggest that signal transduction via angiotensin II/At1r plays important role in regulation of apoptosis of intestinal epithelial cells (enterocytes); At1r may be important in modulation of enterocyte apoptosis as seen in short bowel syndrome. PMID: 23783021
  43. In the infralimbic prefrontal cortex, increased c-fos immunoreactivity was found in response to amphetamine and saline challenge, and both were prevented by the AT receptor blocker. PMID: 24089683
  44. A significantly decreased expression of AT1R in the entire hypothalamic tissue extract of maternal protein restriction (LP) rats at 12 days of age while the expression of the AT2R in 12-day- and 16-week-old LP hypothalamus was significantly increased. PMID: 22936038
  45. Results indicate that CIH induced the elevation of rat blood pressure and aorta AT1R expression PMID: 24033947
  46. This study provides a mechanism by which GRK4, via regulation of arterial AT(1)R expression and function, participates in the pathogenesis of conduit vessel abnormalities in hypertension. PMID: 24218433
  47. AT1 receptors prevent salt-induced vascular dysfunction in isolated middle cerebral arteries of 2 kidney-1 clip hypertensive rats. PMID: 23934707
  48. Overproduction of reactive oxygen species at the early stage of high glucose incubation stimulates p102 synthesis, which in turn up-regulates AT1 receptor expression. PMID: 23376562
  49. AT1R mediates the hyperthyroidism-induced increase in cardiac miRNA-208alpha/alpha-myosin heavy chains levels, while does not influence the reduction in miRNA-208beta/beta-myosin heavy chains levels. PMID: 23623871
  50. Single artery occlusion without significant visible infarct resulted in locally increased ETB, angiotensin type 1 and 5-hydroxytryptamine 1B receptor-mediated contractile responses only in segments located downstream of the occlusion site. PMID: 23988741
  51. Interplay between EGR1 and SP1 is critical for 13-cis retinoic acid-mediated transcriptional repression of angiotensin type 1A receptor. PMID: 23475749
  52. Overexpression of ACE2 gene could inhibit vascular smooth muscle cell proliferation by downregulating AT1 receptor expression and STAT3 phosphorylation. PMID: 22943692
  53. Data show that pinocembrin inhibits AngII-induced aortic ring contraction by suppression of the increase in [Ca(2+)]i and ERK1/2 activation via blocking AT1R. PMID: 23611777
  54. Internalization of activated AT1AR beta-arrestin-mediated vascular smooth muscle cell culture activates the NF-kappaB pathway. PMID: 23576578
  55. Data indicate that mRNA of tissue renin-angiotensin system (tRAS) components angiotensin converting enzyme, Ang II, Ang II receptor type 1, Ang II receptor type 2, and Cathepsin D in intervertebral disc (IVD) was clearly confirmed BY RT-PCR. PMID: 23132538
  56. increased expression of the AT1Ra in SHR NTS may present a counterhypertensive mechanism involving inflammatory/angiogenic cells. PMID: 23547238
  57. Blockage of AT1 receptor increased strength, mass and trabecular connections of ovariectomized rat femurs. PMID: 23087139
  58. Data indicate tjat AT1 receptor blockade completely abrogated AT1-AA-induced TNF-alpha secretion, caspase-3 activation, and cardiomyocyte apoptosis. PMID: 23089979
  59. Down regulation of AT1 and AT2 receptor expression has a negative association with apoptosis of myocardial cells from endotoxemic rats. PMID: 22860435
  60. analysis of mutations on specific conformational changes in the extracellular loop 2 of angiotensin II type 1 receptor PMID: 23139413
  61. analysis of changes in angiotensin II type 1 receptor expression in the rat bladder by bladder outlet obstruction PMID: 22777104
  62. short-term treatment with two AT1 receptor blockers has different effects on diameter of pial arterioles in SHR PMID: 22957022
  63. Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats PMID: 23087176
  64. This study provides novel evidence that acute ethanol intake stimulates renin-angiotensin system activity and induces vascular oxidative stress and redox-signaling activation through AT-dependent mechanisms. PMID: 22982071
  65. Candesartan may protect neurons by decreasing extracellular glutamate immediately after reperfusion and by attenuating oxidative stress via a modulation of the AT1-R signaling that occurs during ischemic insult. PMID: 22661470
  66. Increased GRK5 may regulate AT1R expression in chronic heart failure, and exercise training mitigates AT1R and its pathway components. PMID: 22753221
  67. Brain AT1 receptor appears to be involved in mediating natriuresis induced by cholinergic stimulus. PMID: 21166237
  68. angiotensin type 1 and type 2 receptors are involved in estrogen-induced cell proliferation and vascular endothelial growth factor expression in the rat anterior pituitary PMID: 22645419
  69. Valsartan reduced the incidence of programmed electrical stimulation induced ventricular arrhythmia; this effect was possibly through modulating the myocardial AT1R and Cx43 expression. PMID: 22128836
  70. Ang-(1-7) and Ang II specifically interact with each other on rat renal mesangial cells via activation of their specific receptors, Mas and AT1 receptor respectively. PMID: 22561449
  71. Collectively, these data indicated an important role for AT1-AA stimulated in response to placental ischemia that caused hypertension during pregnancy. PMID: 22498426
  72. allosteric interactions in the homodimers of ATR significantly affect the function of the non-stimulated protomer, and the conserved DRY motif has a crucial role in these interactions. PMID: 22579851
  73. This study suggests the presence of Ang II-mediated brain inflammatory events in diabetes probably mediated by AT1 receptors. PMID: 22464881
  74. Data show that 13-cis-retinoic acid has a glucose- and RAR/RXR-independent mechanism for transcriptional inhibition of AT1. PMID: 22180636
  75. Injection of cholinergic agonist carbachol can enhance the activity of adrenergic neurons and the expression of AT1 receptor in locus coeruleus. PMID: 21186577
  76. insulin sensitivity enhancement after long-term treatment with AT1 blockers is not associated with PPARgamma target gene regulation PMID: 22253423
  77. Ang II-induced hypertension is mediated by an increase in sympathetic nerve activity, which seems to involve up-regulation of AT1 receptors in the rostral ventrolateral medulla and down-regulation of AT1 receptors in the subfornical organ. PMID: 21393361
  78. Visfatin expression was increased mainly through the AT1-R-JAK/STAT pathway in the process of Ang II-induced cardiomyocyte hypertrophy. PMID: 21841463
  79. These results suggested that the modulation of AT-1R/AMPK-MAPK pathway might play crucial roles for the pathogenesis of diabetic cardiomyopathy and it could become an important therapeutic target to ameliorate the diabetic cardiomyopathy. PMID: 22146585
  80. Data demonstrate tissue-specific changes in AT1a-b and AT2 receptor expression after myocardial infarction, and show that MI has a strong influence on the expression of angiotensin receptor type AT1 in the prostate at the protein and mRNA level. PMID: 22038231
  81. Ang II induces ERK1/2 activation, contributing to the downregulation of CAT as well as promoting oxidative stress and adventitial fibroblast phenotypic differentiation in an AT1R-mediated manner. PMID: 21660462
  82. Long-term AT(1) receptor blockade increases the mRNA of the enzymes ACE2 and neprilysin and the MAS receptor. PMID: 21540301
  83. The data suggest a mechanism in which fluid flow directly acts on AT1R, , whereas cyclic strain reduces activation of smad proteins. These results have relevance to the pathogenesis and treatment of heart failure. PMID: 22020089
  84. Data show that knockdown of PHD2, a major isoform of PHDs, by RNA interference reduced AT(1)R expression. PMID: 21825224
  85. RT-PCR revealed that Angiotensin-II infusion induced increased Angiotensin-II type I receptor mRNA levels in the rostral ventrolateral medulla and decreased in subfornical organ. PMID: 21897104
  86. In contrast to observations reported by other authors, the AA genotype of the AGTR1 1166A/C gene polymorphism - much more than other genotypes - was associated with hypertension. PMID: 21657802
  87. These results indicate that medial septal area AT(1) and AT(2) receptors act synergistically in the regulation of urine and sodium excretion induced by arginine vasopressin. PMID: 20663845
  88. AT and AT receptors, NAD(P)H oxidase and PKC pathway are involved in the regulation of hydromineral metabolism and antioxidant enzyme activity induced by angiotensin II. PMID: 20807796
  89. AT1R and AT2R protein expressions in the brainstem, kidney and liver from male foetuses (3 days before birth), male neonates (3 days after birth), male and female adults (8 weeks) and male aged (28 months) rats, were studied. PMID: 20807798
  90. The increased AT1 expression was significantly higher in the large than in the small glomeruli from diabetic and 12(S)-HETE-treated rats. PMID: 21285403
  91. These data strongly indicate that Rab1 modulates pulmonary artery smooth muscle cell function by manipulating AT1R traffic from the endoplasmic reticulum to the Golgi. PMID: 21095238
  92. Blockade of brain AT receptor can down-regulate the activity of adrenergic neurons in the rostral ventrolateral medulla. PMID: 21319377
  93. Results suggest that endogenous ANG II acts on AT1 receptors in the PVN to mediate the reduction in RBF induced by hyperthermia. PMID: 21123758
  94. The imbalance in angiotensin II type 1 receptor and angiotensin II Type 2 receptor expression is associated with an inflammatory process that contributes to renal injury in adult spontaneously hypertensive rats and to the development of hypertension. PMID: 21084406
  95. TNF-alpha in the paraventricular nucleus modulates neurotransmitters and the expression of AT1 receptor, which could account for exaggerated sympathetic activity in heart failure. PMID: 21135513
  96. Results reveal an important interaction between the dopaminergic and local renin-angiotensin system, including AT1 and 2 receptors, in the basal ganglia, which may be a major factor in the progression of Parkinson's disease. PMID: 20964730
  97. AT(1)R and alpha1D -adrenoceptor may form heterodimers, and may play a role in preeclampsia. PMID: 20102360
  98. Renal AT(1) (p < 0.0001) and AT(2) (p < 0.0001) receptor protein expression were significantly lower in low protein rats from E18 to day 10 PMID: 20606474
  99. Paraventricular AT1a receptors are critical for short-term circulating ANG II-mediated elevations of blood pressure. Suppression of AT(1a)R expression by single administration of shRNA can interfere with short-term actions of ANG II. PMID: 20702798
  100. This study demonstrated that miR-155 could have an important role in regulating adventitial fibroblast differentiation and contribute to suppression of AT(1)R expression. PMID: 20735984

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Is expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal gland, and vascular smooth muscle cell.
Database Links

KEGG: rno:24180

STRING: 10116.ENSRNOP00000034687

UniGene: Rn.9814

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