Rat angiotensin Ⅱ receptor type 1(AT1R)ELISA Kit

Instructions
Code CSB-E13746r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name angiotensin II receptor, type 1
Alternative Names Agtr1 ELISA kit; Agtr1a ELISA kit; At1aType-1A angiotensin II receptor ELISA kit; Angiotensin II type-1A receptor ELISA kit; AT1A ELISA kit
Abbreviation AGTR1
Uniprot No. P25095
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AT1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %87-97
1:2Average %97
Range %95-102
1:4Average %101
Range %95-105
1:8Average %94
Range %91-98
Recovery
The recovery of rat AT1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9995-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.931 2.874 2.903 2.757
25002.398 2.455 2.427 2.281
12501.799 1.740 1.770 1.624
6250.923 0.984 0.954 0.808
3120.545 0.589 0.567 0.421
1560.477 0.486 0.482 0.336
780.280 0.259 0.270 0.124
00.141 0.151 0.146
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Gene References into Functions
  1. Following restraint stress, AT1 receptors in the pre-limbic cortex modulate the acute restraint stress-evoked pressor response. PMID: 28695320
  2. A possible reduction in CB1R functionality, coupled with a hyperfunctional AT1R in the brainstem, could well be significant factors in the development of hypertensive states. AT1R-mediated phosphorylation of CB1R could be critical for impaired cerebellar development characterized by a hyperactive RAS PMID: 28887229
  3. In the aged offspring of prenatal high sucrose exposure rats, mRNA and protein expressions of AT1R gene in both large and small blood vessels were significantly increased. PMID: 27818235
  4. AT1R activation results in translocation of RGS5 toward the plasma membrane, limiting AT1R-mediated vasoconstriction through its role in Gq/11 protein-dependent signaling. PMID: 29061726
  5. The present studies support growing evidence for novel modes of activation of the AT1 R in arterioles and suggest that mechanically activated AT1 R generates diacylglycerol, which in turn activates PKC which induces the actin cytoskeleton reorganization that is required for pressure-induced vasoconstriction PMID: 27531064
  6. Altered expression of AT1 and AT2 receptors with aging may induce mitochondrial dysfunction, the main risk factor for neurodegeneration. PMID: 27763643
  7. In this study, we demonstrate that vitamin D improved many factors associated with PE and reduced blood pressure in a hypertensive model without PE features, indicating that vitamin D could be beneficial for various hypertensive disorders of pregnancy. PMID: 27903510
  8. Results demonstrated that uric acid can stimulate p38 MAPK, p44/42 MAPK and PDGFRbeta phosphorylation in a time- and concentration-dependent manner. Furthermore, treatment of VSMCs with the angiotensin II type I receptor (AT1R) inhibitor losartan suppressed p38 MAPK and p44/42 MAPK induction by uric acid. PMID: 27400779
  9. the AT1 expression in neuronal subpopulation of lower lumbar dorsal root ganglia under normal conditions and its alteration in neuropathic pain model, is reported. PMID: 27319015
  10. results suggest that the AT1 receptor is involved in development of hypertension and cardiac fibrosis PMID: 27085217
  11. Data indicate the effect of angiotensin II receptors' blockade on the macrophage infiltration and histone modifications occurring at the promoter region of monocyte chemoattractant protein (MCP-1) and transforming growth factor-beta (TGF-beta1). PMID: 27693081
  12. Vinegar activates AMPK by increasing AMP/ATP ratios, thereby increases PGC-1alpha and PPARgamma expressions, and inhibits AT1R expression in spontaneously hypertensive rats. PMID: 26476634
  13. AT1R induces the redox-sensitive signaling pathway in the brain stem. PMID: 26575857
  14. The prothrombotic effect of aldosterone is partially mediated by the AT1 receptor. PMID: 26709040
  15. aldosterone induced an inflammatory response in rat mesangial cells cultured in high-glucose media via the AT1a and AT2 pathways. PMID: 24464860
  16. in diabetes, AT1R increases prostaglandins E2 (PGE2) generation and reduces conversion of PGE2 to PGF2alpha with the progression of diabetes. PMID: 26195268
  17. Previously unappreciated functions of AT1aR helix 8 and novel mechanisms governing the cell surface transport and function of AT1aR. PMID: 26342563
  18. Results support functional interactions between AT1 receptors and TLR4 in mediating ANG II-dependent microglial activation and oxidative stress within the hypothalamic paraventricular nucleus. PMID: 26637556
  19. Endogenous hydrogen sulfide reduced the liver expression levels of AGTR1, which may be associated with the delayed progression of hepatic fibrosis. PMID: 26034979
  20. The role of angiotensin II type I receptor (AT1-AA) in hypertension and preeclampsia in rats is reported. PMID: 26310940
  21. These findings indicate that biased signaling of the beta-arrestin pathway through the AT1 receptor inhibits NHE3 activity in the proximal tubule at least in part due to changes in NHE3 subcellular localization. PMID: 26246427
  22. AVP-induced increase in AQP2 is blunted in heart failure during cardiac remodeling and is associated with decreased AT1R abundance in rat kidney. PMID: 25658446
  23. in rats with post-infarction heart failure the regulation of blood pressure by AT1Rs depends on centrally acting endogenous TNF-alpha PMID: 25481865
  24. Heightened AT1aR expression in spontaneously hypertensive rats is related to the AT1aR promoter hypo-methylation. PMID: 25596947
  25. Clathrin-dependent internalization of the angiotensin II ATA receptor links receptor internalization to COX-2 protein expression in rat aortic vascular smooth muscle cells PMID: 25542758
  26. our results point out multiple actions of Ang II in glioma pathogenesis and reveal the participation of both Ang II receptors in the regulation of genes relevant for glioma progression. PMID: 25365520
  27. Vascular oxidative can upregulate AT1R, via mechanisms involving NFkappaB, and contribute to the development of hypertension. PMID: 25198883
  28. These findings suggest that an interaction between AT1-R and IL-1beta in the paraventricular nucleus contributes to progression in heart failure. PMID: 24955935
  29. We conclude that Ang II pretreatment, as a trigger, activated the AT1R/HIF-1alpha/ACE axis that then mediated Ang II-induced VEGF synthesis in MSCs. PMID: 25401104
  30. Venthamarai chooranam, a polyherbal Siddha medicine, alleviates hypertension via ATR and eNOS signaling pathway in 2K1C hypertensive rats PMID: 24719376
  31. Hyperhomocysteinemia accelerates collagen accumulation in the adventitia of balloon-injured rat carotid arteries via angiotensin II type 1 receptor. PMID: 25350112
  32. The Ang II AT1 receptors in Central nucleus of the amygdala are involved in the anxiety state induced by stress in the fear-potentiated plus-maze behaviour. PMID: 25003108
  33. Western blot analysis revealed that AT1 expression increased in the control group during aging while AT2 and Mas remained unchanged PMID: 24819472
  34. Arrestins coordinate AT1 receptor regulation of ERK1/2 and Akt activity and stimulate protein translation via both Akt-mTOR-p70/85S6K and ERK1/2-p90RSK pathways. PMID: 25081544
  35. A counterregulatory interaction between dopamine and the angiotensin receptor was observed. PMID: 24529758
  36. Serum levels of ghrelin are significantly positively correlated with Ang II in chronic heart failure (CHF) patients and that ghrelin can inhibit Ang II-induced cardiomyocyte apoptosis by down-regulating AT1R, thereby playing a role in preventing HF. PMID: 24465706
  37. angiotensin signaling through Agtr1a in the subfornical organ contributes to increased water intake, but not dilutional hyponatremia, during congestive liver failure. PMID: 25009217
  38. The enhanced downregulation of the AT1-B receptors in the renin-negative SMCs in the efferent arterioles demonstrates that the regulation of the glomerular filtration rate by the pre- and postglomerular arterioles is changed in diabetes PMID: 24587998
  39. 2-methoxyestradiol down-regulation of angiotensin AT1 receptor is dependent on endoplasmic reticulum membrane-associated GRP30. PMID: 24262995
  40. Interaction between AT1 receptor and nuclear factor (NF)-kappaB in the paraventricular nucleus contributes to oxidative stress in heart failure patients. PMID: 23877628
  41. Our results are the first to show that activation of the brain RAS after surgery contributes to POCD in aged rats. PMID: 24814703
  42. Data suggest that signal transduction via angiotensin II/At1r plays important role in regulation of apoptosis of intestinal epithelial cells (enterocytes); At1r may be important in modulation of enterocyte apoptosis as seen in short bowel syndrome. PMID: 23783021
  43. In the infralimbic prefrontal cortex, increased c-fos immunoreactivity was found in response to amphetamine and saline challenge, and both were prevented by the AT receptor blocker. PMID: 24089683
  44. A significantly decreased expression of AT1R in the entire hypothalamic tissue extract of maternal protein restriction (LP) rats at 12 days of age while the expression of the AT2R in 12-day- and 16-week-old LP hypothalamus was significantly increased. PMID: 22936038
  45. Results indicate that CIH induced the elevation of rat blood pressure and aorta AT1R expression PMID: 24033947
  46. This study provides a mechanism by which GRK4, via regulation of arterial AT(1)R expression and function, participates in the pathogenesis of conduit vessel abnormalities in hypertension. PMID: 24218433
  47. AT1 receptors prevent salt-induced vascular dysfunction in isolated middle cerebral arteries of 2 kidney-1 clip hypertensive rats. PMID: 23934707
  48. Overproduction of reactive oxygen species at the early stage of high glucose incubation stimulates p102 synthesis, which in turn up-regulates AT1 receptor expression. PMID: 23376562
  49. AT1R mediates the hyperthyroidism-induced increase in cardiac miRNA-208alpha/alpha-myosin heavy chains levels, while does not influence the reduction in miRNA-208beta/beta-myosin heavy chains levels. PMID: 23623871
  50. Single artery occlusion without significant visible infarct resulted in locally increased ETB, angiotensin type 1 and 5-hydroxytryptamine 1B receptor-mediated contractile responses only in segments located downstream of the occlusion site. PMID: 23988741

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Is expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal gland, and vascular smooth muscle cell.
Database Links

KEGG: rno:24180

STRING: 10116.ENSRNOP00000034687

UniGene: Rn.9814

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