Rat annexin I (ANX-I) ELISA Kit

Instructions
Code CSB-E15811r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name annexin I (ANX-I)
Abbreviation ANX-I
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.63 ng/mL-40 ng/mL
Sensitivity 0.16 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ANX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %86-97
1:2Average %103
Range %99-107
1:4Average %91
Range %83-95
1:8Average %94
Range %90-98
Recovery
The recovery of rat ANX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
EDTA plasma (n=4)9286-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.511 2.313 2.412 2.264
201.740 1.844 1.792 1.644
101.187 1.097 1.142 0.994
50.654 0.632 0.643 0.495
2.50.440 0.425 0.433 0.285
1.250.349 0.337 0.343 0.195
0.630.236 0.224 0.230 0.082
00.148 0.147 0.148
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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