Rat cluster Of differentiation,CD44 ELISA Kit

Instructions
Code CSB-E10643r
Size 96T,5×96T,10×96T
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Product Details

Target Name CD44 molecule (Indian blood group)
Alternative Names Cd44CD44 antigen ELISA kit; Extracellular matrix receptor III ELISA kit; ECMR-III ELISA kit; GP90 lymphocyte homing/adhesion receptor ELISA kit; HUTCH-I ELISA kit; Hermes antigen ELISA kit; Hyaluronate receptor ELISA kit; Phagocytic glycoprotein 1 ELISA kit; PGP-1 ELISA kit; Phagocytic glycoprotein I ELISA kit; PGP-I ELISA kit; CD antigen CD44 ELISA kit
Abbreviation CD44
Uniprot No. P26051
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 82-94
1:2 Average % 101
Range % 95-104
1:4 Average % 97
Range % 92-102
1:8 Average % 88
Range % 83-97
Recovery
The recovery of rat CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 81-91
EDTA plasma (n=4) 99 95-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.294 2.220 2.257 2.151
200 1.746 1.644 1.695 1.589
100 1.120 1.164 1.142 1.036
50 0.644 0.610 0.627 0.521
25 0.384 0.406 0.395 0.289
12.5 0.259 0.255 0.257 0.151
6.25 0.181 0.195 0.188 0.082
0 0.103 0.109 0.106  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function.
Gene References into Functions
  1. The findings indicate for the first time that regulation of Rac1 activity is responsible for hyaluronidase and CD44-driven morphological changes of astrocytes. PMID: 27163367
  2. results suggest that the AT1 receptor is involved in development of hypertension and cardiac fibrosis. Selective activating ACE2/eNOS and inhibiting CD44/HA interaction might be considered as the therapeutic targets for attenuating Ang II induced deleterious cardiovascular effects PMID: 27085217
  3. Results define a clinically relevant role for signaling by the hyaluronan receptor, CD44, in increased responsiveness to mechanical stimulation PMID: 26996509
  4. Peptide inhibitors of CD44v6 isoforms block tumor growth and metastasis in several independent models of pancreatic cancer. PMID: 26597578
  5. Expression of CD44 followed the same temporal pattern as hyaluronic acid deposition in a bladder regeneration model. PMID: 25253654
  6. Results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease. PMID: 25300795
  7. Data show that hyaluronan receptor CD44 undergoes co-internalization with hyaluronan endocytosis. PMID: 25733665
  8. release of the CD44 intracellular domain into the cytoplasm of cells such as chondrocytes exerts a competitive or dominant-negative effect on the function of full-length CD44. PMID: 23884413
  9. CD44 is a key molecule in the migration of OPCs toward the focal inflammatory demyelinated lesion induced by zymosan PMID: 23280959
  10. after experimental TBI, hippocampal neurons grew well on an OPN substrate with growth comparable to that seen on laminin, but showed a higher degree of primary neurites; growth on OPN was mediated through beta1 intregrins and CD44. T PMID: 22692550
  11. Upregulation of hyaluronan and its binding receptors CD44 and LYVE-1 are involved in interstitial fibrosis in chronic cyclosporine-induced renal injury. PMID: 20470282
  12. Results describe how CD44 regulates the motility and invasive growth of B35 neuroblastoma cells into a hyaluronan-rich environment. PMID: 20056122
  13. Data show that Hyal2, through direct interactions with CD44 and possibly some pericellular hyaluronidase activity suppresses the formation or the stability of the glycocalyx, modulates ERM-related cytoskeletal interactions, and diminishes cell motility. PMID: 19783662
  14. Data show that neither CD44v nor exosomes alone suffice for (pre)metastatic niche formation. PMID: 19794968
  15. role of colocalization with osteopontin in migration, cell fusion, and resorption in osteoclasts PMID: 12162503
  16. Data show that CD44 antigen is upregulated on all migrating cells and on newly formed blood vessels at the onset of the foreign body reaction. PMID: 12579564
  17. Microarray analysis and RNA silencing link fra-1 to the expression of this antigen and c-met expresssion in mesothelioma. PMID: 12839939
  18. Cd44 variant isoforms associate with tetraspanins and EpCAM. PMID: 15212938
  19. Cd44 expression identifies astrocyte-restricted precursor cells. PMID: 15531362
  20. The helper and cytotoxic subsets of T cells, as well as B cells, display the increased expression of CD44, intercellular adhesion molecule 1 and lymphocyte function associated antigen 1 PMID: 15624701
  21. migration of rat hepatic stellate cells requires hyaluronic acid and CD44v6 PMID: 15691832
  22. NCoR, GAF, and TR interact with the CD44 negative T(3)-responsive element to enhance basal transactivation, whereas T(3) induces the remodeling of chromatin structure for repression PMID: 15701601
  23. role of CD44-hyaluronan interaction in mesenchymal stem cell migration in the extracellular matrix PMID: 16306150
  24. CD44 is a specific marker of small hepatocytes. PMID: 16580085
  25. CD44 is probably involved in early leukocyte infiltration, in tubular regeneration, and in macrophage activity in the rat kidney after ischemia/reperfusion injury. PMID: 16786159
  26. The expression pattern of CD44 was studied in the rat testis following ischemia/reperfusion (I/R) injury to elucidate the possible role of the CD44 adhesion molecule in acute experimental testicular torsion. PMID: 16891795
  27. The association of ezrin, radixin and moesin proteins to the coreceptor is absolutely required to mediate the hepatocyte growth factor-dependent activation of Ras by the guanine nucleotide exchange factor Sos. PMID: 17065554
  28. CD44 has a pivotal role in the trans-endothelial migration of NPCs across brain endothelial cells PMID: 18450824
  29. CD44-hyaluronan interaction, by enhancing biliary proliferation, may play a pathogenic role in the development of cholestatic liver diseases. PMID: 18556418
  30. Data show that the rapid and transient induction of CD44 occurrs in activated microglia/macrophages following transient forebrain ischemia. PMID: 18638458
  31. CD44 upregulation in neurons is affected by local chondroitin sulfate proteoglycan expression. PMID: 18694596
  32. CD44 variant isoforms promote metastasis formation by a tumor cell-matrix cross-talk that supports adhesion and apoptosis resistance. PMID: 19208744
  33. CD44 may play an important role in the development of airway inflammatory in asthma. PMID: 19222954
  34. Importantly, TSC activation upon ALS-like neurodegeneration results in significant increase in molecular proximity of CD44 and ErbB3, which may have an impact on glial plasticity at the NMJ. PMID: 19385056
  35. Results suggest that Tra2beta may regulate cellular oxidative response by changing alternative splicing of distinct genes including CD44. PMID: 19439532

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Database Links

UniGene: Rn.1120

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