Rat creatine kinase BB isoenzyme (CK-BB) ELISA kit

Instructions
Code CSB-EQ027522RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name creatine kinase BB isoenzyme (CK-BB)
Abbreviation CK-BB
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 mU/mL-40 mU/mL
Sensitivity 0.156 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %97
Range %92-101
1:4Average %86
Range %82-90
1:8Average %102
Range %98-106
Recovery
The recovery of rat CK-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-98
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1OD2AverageCorrected
402.152 2.247 2.200 2.025
201.565 1.621 1.593 1.418
101.243 1.186 1.215 1.040
50.997 0.954 0.976 0.801
2.50.725 0.753 0.739 0.564
1.250.586 0.562 0.574 0.399
0.6250.339 0.325 0.332 0.157
00.171 0.179 0.175
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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