Rat cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Code CSB-E09243r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cross linked N-telopeptide of type Ⅰ collagen,NTX
Alternative Names cross linked N-telopeptide of type 1 collagen,NTX,cross linked N-telopeptide of type I collagen,NTX
Abbreviation NTX
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 4.7 nM BCE-300 nM BCE
Sensitivity 1.17 nM BCE
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 92  
Range % 88-98  
1:10 Average % 96  
Range % 91-102  
1:20 Average % 99  
Range % 92-104  
1:40 Average % 94  
Range % 90-98  
The recovery of rat NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 93-102  
EDTA plasma (n=4) 93 87-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nM BCE OD1 OD2 Average Corrected  
300 2.712 2.799 2.756 2.571  
150 1.967 1.989 1.978 1.793  
75 1.458 1.467 1.463 1.278  
37.5 1.053 1.069 1.061 0.876  
18.7 0.702 0.717 0.710 0.525  
9.4 0.466 0.498 0.482 0.297  
4.7 0.291 0.281 0.286 0.101  
0 0.183 0.186 0.185    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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