Rat cystathionine β-synthase(CBS) ELISA Kit

Code CSB-E13323r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P32232
Lead Time 3-5 working days
Protein Biological Process 2 Amino-acid biosynthesis and metabolism
Abbreviation CBS
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name cystathionine-beta-synthase
Alias HIP4, OTTHUMP00000109415|OTTHUMP00000109416|OTTHUMP00000109418|beta-thionase|cystathionine beta-synthase|methylcysteine synthase|serine sulfhydrase
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Amino-acid biosynthesis
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.
HGNC 1550
RGD 2287
MGI 88285
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 103
Range % 94-107
1:2 Average % 112
Range % 103-117
1:4 Average % 94
Range % 84-98
1:8 Average % 98
Range % 87-102
Recovery
The recovery of rat CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 83-94
EDTA plasma (n=4) 85 84-87
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.325 2.248 2.287 2.094
1000 1.496 1.426 1.461 1.268
500 1.030 1.097 1.064 0.871
250 0.692 0.723 0.708 0.515
125 0.449 0.467 0.458 0.265
62.5 0.377 0.385 0.381 0.188
31.25 0.300 0.289 0.295 0.102
0 0.195 0.191 0.193  
References
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Function Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons
Subcellular Location Cytoplasm, Nucleus
Protein Families Cysteine synthase/cystathionine beta-synthase family
Tissue Specificity Expressed in liver, kidney and brain. Highly expressed in the hippocamus and cerebellum.
Database Links

KEGG: rno:24250

STRING: 10116.ENSRNOP00000039968

UniGene: Rn.87853

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