Rat immunoglobulin D(IgD) ELISA kit

Instructions
Code CSB-EQ027960RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name immunoglobulin D(IgD)
Abbreviation IgD
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 1.22 pg/mL-5000 pg/mL
Sensitivity 1.22 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 94
Range % 85-103
1:2000 Average % 95
Range % 87-100
1:4000 Average % 90
Range % 86-100
1:8000 Average % 87
Range % 81-96
Recovery
The recovery of rat IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-107
EDTA plasma (n=4) 95 90-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.356 2.397 2.377 2.274
1250 2.202 2.185 2.194 2.091
312.5 1.664 1.692 1.678 1.575
78 1.241 1.255 1.248 1.145
19.5 0.799 0.784 0.792 0.689
4.88 0.425 0.433 0.429 0.326
1.22 0.275 0.280 0.278 0.175
0 0.101 0.105 0.103  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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