Rat interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) (IL12A) ELISA kit

Instructions
Code CSB-EL011586RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Alternative Names Il12a ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
Abbreviation IL12A
Uniprot No. Q9R103
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL12A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 86-93
1:2 Average % 88
Range % 81-95
1:4 Average % 97
Range % 93-104
1:8 Average % 101
Range % 98-106
Recovery
The recovery of rat IL12A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 94-109
EDTA plasma (n=4) 93 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.868 2.785 2.827 2.737
400 2.247 2.251 2.249 2.159
200 1.466 1.452 1.459 1.369
100 0.818 0.858 0.838 0.748
50 0.439 0.470 0.455 0.365
25 0.277 0.294 0.286 0.196
12.5 0.189 0.180 0.185 0.095
0 0.092 0.088 0.090  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. Data show that endogenous interleukins IL-27 and IL-35 (Ebi3 and Il-12p35 subunits) had inflammatory roles and insulin like growth factor -1 (IGF-1) had no effect. PMID: 26994309
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: rno:84405

STRING: 10116.ENSRNOP00000012832

UniGene: Rn.207199

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