Rat leukotriene B5(LT-B5) ELISA kit

Code CSB-EQ027966RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leukotriene B5(LT-B5)
Abbreviation LT-B5
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %91-103
1:2Average %89
Range %84-95
1:4Average %95
Range %90-99
1:8Average %84
Range %80-88
The recovery of rat LT-B5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)106100-115
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2002.769 2.652 2.711 2.585
1002.413 2.376 2.395 2.269
501.707 1.844 1.776 1.650
250.882 0.816 0.849 0.723
12.50.475 0.447 0.461 0.335
6.250.261 0.271 0.266 0.140
3.120.198 0.189 0.194 0.068
00.130 0.121 0.126
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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