Rat leukotriene B5(LT-B5) ELISA kit

Code CSB-EQ027966RA
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Abbreviation LT-B5
Species Rat
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-3h
Sample Volume 50-100ul
Detection Wavelength 450nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %91-103
1:2Average %89
Range %84-95
1:4Average %95
Range %90-99
1:8Average %84
Range %80-88
Recovery
The recovery of rat LT-B5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)106100-115
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.769 2.652 2.711 2.585
1002.413 2.376 2.395 2.269
501.707 1.844 1.776 1.650
250.882 0.816 0.849 0.723
12.50.475 0.447 0.461 0.335
6.250.261 0.271 0.266 0.140
3.120.198 0.189 0.194 0.068
00.130 0.121 0.126

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