Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
The recovery of rat LT-B5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Average % Recovery
EDTA plasma (n=4)
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.