Rat leukotriene B5(LT-B5) ELISA kit

Code CSB-EQ027966RA
See More Details
Product Type ELISA kit
Size 96T,5×96T,10×96T
Abbreviation LT-B5
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Availability 3-5 working days
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %91-103
1:2Average %89
Range %84-95
1:4Average %95
Range %90-99
1:8Average %84
Range %80-88
Recovery
The recovery of rat LT-B5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)106100-115
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.769 2.652 2.711 2.585
1002.413 2.376 2.395 2.269
501.707 1.844 1.776 1.650
250.882 0.816 0.849 0.723
12.50.475 0.447 0.461 0.335
6.250.261 0.271 0.266 0.140
3.120.198 0.189 0.194 0.068
00.130 0.121 0.126
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

LT-B5 ELISA Kit

LT-B5 ELISA Kit for Homo sapiens (Human)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.