Sheep Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) ELISA kit

Code CSB-EL001631SH
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 7-14 working days
Abbreviation ALPL
Target Name alkaline phosphatase, liver/bone/kidney
Alias AP-TNAP, FLJ40094, FLJ93059, HOPS, MGC161443, MGC167935, TNAP, TNSALP, OTTHUMP00000002972|alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphomonoesterase|glycerophosphatase|liver/bone/
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
HGNC 438
RGD 2100
MGI 87983
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep ALPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 102
Range % 97-106
1:2 Average % 95
Range % 91-99
1:4 Average % 92
Range % 85-96
1:8 Average % 93
Range % 86-97
Recovery
The recovery of sheep ALPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-96
EDTA plasma (n=4) 104 97-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
1000 1.945 1.854 1.900 1.749
500 1.476 1.427 1.452 1.301
250 1.124 1.074 1.099 0.948
125 0.776 0.784 0.780 0.629
62.5 0.573 0.581 0.577 0.426
31.2 0.357 0.361 0.359 0.208
15.6 0.248 0.245 0.247 0.096
0 0.152 0.150 0.151  
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