Sheep Interleukin-12 subunit beta(IL12B) ELISA kit

Instructions
Code CSB-EL011587SH
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Interleukin-12 subunit beta(IL12B)
Alternative Names IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
Abbreviation IL12B
Uniprot No. P68220
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL12B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 80-105
1:2 Average % 92
Range % 88-99
1:4 Average % 95
Range % 88-100
1:8 Average % 97
Range % 83-106
Recovery
The recovery of sheep IL12B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 85-102
EDTA plasma (n=4) 96 88-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.752 2.715 2.734 2.530
800 2.286 2.302 2.294 2.090
400 1.562 1.543 1.553 1.349
200 0.792 0.803 0.798 0.594
100 0.468 0.451 0.460 0.256
50 0.357 0.361 0.359 0.155
25 0.275 0.286 0.281 0.077
0 0.203 0.205 0.204  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
  2. Local intraocular production of IL-12 p40 (but not IL-4) prolongs corneal graft survival significantly PMID: 16081789
Subcellular Location Secreted
Protein Families Type I cytokine receptor family, Type 3 subfamily
Database Links

KEGG: oas:443472

UniGene: Oar.721

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