Sheep Interleukin-5(IL5) ELISA kit

Instructions
Code CSB-EL011662SH
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 5 (colony-stimulating factor, eosinophil)
Alternative Names IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation IL5
Uniprot No. Q28586
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-90
1:2 Average % 91
Range % 85-97
1:4 Average % 89
Range % 85-92
1:8 Average % 100
Range % 97-103
Recovery
The recovery of sheep IL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 82-91
EDTA plasma (n=4) 93 87-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.492 2.600 2.546 2.444
500 2.019 2.001 2.010 1.908
250 1.591 1.632 1.612 1.510
125 1.068 1.102 1.085 0.983
62.5 0.680 0.675 0.678 0.576
31.25 0.392 0.384 0.388 0.286
15.6 0.250 0.236 0.243 0.141
0 0.101 0.103 0.102  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Gene References into Functions
  1. Pelibuey breed in grazing areas exhibited different expression of IL-5 and IL-6 obtained from peripheral blood mononuclear cells against Haemonchus contortus, suggesting the importance of these cytokines in regulating the nematode infection. PMID: 26094646
Subcellular Location Secreted
Protein Families IL-5 family
Database Links

KEGG: oas:443350

UniGene: Oar.684

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