Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Home
Kit
ELISA Kit
Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

Citations (10) Protocol

Code	CSB-E13853Sh
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Citations (10)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

tumor necrosis factor (TNF superfamily, member 2)

Alternative Names

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit

Abbreviation

TNF

Uniprot No.

P23383

Species

Ovis aries (Sheep)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.125 pg/mL-50 pg/mL

Sensitivity

3.125 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-94
1:8	Average %	102
	Range %	98-109

Recovery

The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	96	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
50	0.161	0.165	0.163
25	0.308	0.324	0.316
12.5	0.551	0.512	0.532
6.25	1.012	0.982	0.997
3.125	2.054	2.011	2.033

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Sheep TNF ELISA Kit was designed for the quantitative measurement of Sheep TNF protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.125 pg/mL-50 pg/mL and the sensitivity is 3.125 pg/mL.

Impact of Vitamin D Supplement on Reproductive Performance in Pre-mating Ewes: Presumptive Regulatory Role of Some Inflammatory Cytokines and Serum Biomarkers GA El-Amrawi,Journal of Veterinary Sciences,2023
Immunological response of lame sheep to Clinical Interdigital Dermatitis and Footrot: Procalcitonin, Acute phase proteins, and Pro-inflammatory Cytokines W El-Deeb,Comparative immunology, microbiology and infectious diseases,2022
Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics N Nasreldin,Animal Reproduction Science,2020
Peripheral and placental immune responses in sheep after experimental infection with Toxoplasma gondii at the three terms of gestation Castano P, et al,Veterinary Research,2019
Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion El-Deeb W, et al,Comparative Immunology, Microbiology and Infectious Diseases,2019
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al,PeerJ,2016
Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al,Comparative Clinical Pathology,,2015
Comparative Efficacy of the Steroids Administered by Inhalation and Parenteral Route in Lambs with Experimentally Induced Endotoxemia Akin Kirbas. et al,International Journal of Veterinary Science,2015
The proteome of Hypobaric Induced Hypoxic Lung: Insights from Temporal Proteomic Profiling for Biomarker Discovery Ahmad Y. et al,Sci Rep,2015

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Function
(From Uniprot)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene References into Functions

The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis PMID: 24995301
TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals. PMID: 24718080
Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21. PMID: 20603027
These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363

Hide All

Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.

Protein Families

Tumor necrosis factor family

Database Links

KEGG: oas:443540

UniGene: Oar.455

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TWIK2 channel mediates inflammation, study finds Osteoprotegerin (OPG): What is Its Potential as a Biomarker or Therapeutic Target? What Are Histones: Unraveling Their Critical Roles in Disease Pathogenesis Multiple Myeloma, One of High-Risk Factors for COVID-19 The True Face of Tumor Suppressor Gene p53 Two Types of Macrophages: M1 and M2 Macrophages An Overview of Cell Death: Necrosis and Apoptosis TNFRSF9: A Potent Immune-Stimulator in Cancer TNFR1: A Multifaceted Molecule in Cancer, Another Promising Therapeutic Target for COVID-19 Cellular Senescence and Its Markers TFPI: a Natural Anticoagulant Protein, Future Therapies for Hemophilia Chemokine Receptor CCR4: the Key Role of CCR4+Treg Cells in Cancer Immunotherapy DT3C, a Powerful Tool for ADC Drug Internalization In Vitro CLDN6: A "New Favorite" in Targeted Cancer Therapy after CLDN 18.2! Intestinal Mucosal Barrier Bodyguard --ALPi GPRC5D: Newly Efficient GPRC5D CAR-T or Bispecific Antibody Therapy to Fight Multiple Myeloma (MM) Dual Role of TNFRSF14 in Immunology With the High Potential in Cancer Immunotherapy, CD40 Is Triggering Attention CD14, A Membrane Receptor for Bacterial Lipopolysaccharides Surface Markers That Help You Identify Lymphocytes Cell Differentiation Determines the Fate of Cells CAR-T Cell Therapy- An Useful Treatment To Cancer CLDN4: A Potential Prognostic Indicator and Therapeutic Target in Tumors！ Differentiation of Th17 Cells Microglial Markers: Insights into Microglial Activation and Function The Overview of Fc Receptors- Classification, Related Signaling Pathways and Application DSG2: a Vital Member of Desmosomal Cadherin Family, an Exciting New Target for Cardiomyopathy, Adenovirus, and Tumors! Surface Markers of Mesenchymal Stem Cells Interleukin 4 receptor (IL-4R/IL-4Ra): a Multifunctional Target of Cytokines, Suggesting Diverse Potential in Asthma, Rhinitis, Viral, and Tumor Research! Stem Cell-What a Miraculous Resource in Human Body IL-6-Do You Really Know That? CEACAM5: A Promising Member of CEACAM Family, A Potential Target for ADC Drug Research! Nanobody, what a Powerful Novel Antibody! CD147, a New Target of SARS-CoV-2 Invasion LPS/TLR4 Signal Transduction Pathway What You Have to Know about Cytokine Storm and Virus Infection Toll-like Receptors--The Eye of Innate Immunity A Comprehensive Exploration into Amyotrophic Lateral Sclerosis What Are Immune Checkpoints? What Is Immune Checkpoint Therapy? What Are Immune Checkpoint Inhibitors? What Are Immune Checkpoint Proteins? Focus on Emerging Therapeutic Targets BCMA What Are Immune Checkpoint Pathways? CD20 Monoclonal Antibodies Drugs: There is an Explosion of New mAbs Against CD20! Tumor Necrosis Factor Receptor Superfamily and Apoptosis VSIG4: a Prominent B7 Family Costimulatory Molecule or Complement Receptor in Cancers and Inflammatory Diseases! Monoclonal Antibody Drugs Overview SLC39A6/ZIP6: a Key Member of Zinc Transporters, a Novel Targeting Antigen of ADC! What You Have To Know about Lung Cancer The Overview of Hippo Signaling Pathway How do Th1 and Th2 Cells Differentiate? Identify Treg Cells. You Can Do This. What A Paradoxical TNF Signaling Pathway-Based on Different Receptors What Essential Biological Knowledge is Needed for Hepatocellular Carcinoma Research? What Are the Effects of Abnormalities in the ALK Gene SIGIRR: A Novel Member of the TIR Superfamily Acts as the Anti-Inflammatory 'Brake' to Control Immune Response in Various Diseases! Mesothelin: A Rising Star of Cancer Immunotherapy CD70 (CD27L/TNFSF7): a TNF Receptor Member, a “Star” Target for Clinical Research in Systemic Lupus Erythematosus (SLE), Arthritis, and Tumors! Does TNF Superfamily Inhibit Cancer or Promote Its Development? An Overview of CD226 and Its Promising Role in Cancer Immunotherapy CD Antigens - From Leucocytes Surface Markers to Diagnostic Markers for Cancer Latest Changes in Journal Impact Factor TM4SF1: a Member of the Transmembrane-4-Superfamily (L6), a New Vascular Therapeutic Target? Surface Markers of Natural Killer Cells Mucin-17/MUC17: An Unexpected Target for Tumor Immune Therapy, Notably Gastric Cancer! The Overview of Chemokine IL-17A: A Major Cytokine of the IL-17 Family, a Key Inflammation Target in Skin Diseases, or Inflammation-Associated Cancers! The Overview of Colony Stimulating Factors Do You Really Know Neurons and Glia Cells in Nerve System? Apoptosis mediated by death receptor CD69: a Classical Early Marker of Lymphocyte Activation, a Pivotal Regulator of Hematologic Diseases and Autoimmune Disorders! PD-1/PD-L1, What a Powerful Immune Checkpoint Cadherin-17 (CDH17): A Viable Target in Bispecific Antibodies or CAR-T Therapy to Fight Many Gastrointestinal Cancers! The Overview of Growth Factor Tumor Necrosis Factor (TNF) The Overview of Interferon Tumor markers The Overview of Interleukin The Overview of Organelles The Overview of Autophagy The overview of Wnt signaling pathway The overview of TGF-β signaling pathway Chemokine Receptor CCR6: the Specific Receptor for CCL20, a Potential Target for Drug Research in Inflammatory Diseases and Gastrointestinal Cancer! IL2RA (CD25): A Specific Marker of Tregs Functions as Both Immune Modulators and Targeted Agents in Various Diseases! Bispecific Antibody Overview: From Definition to Application IGFL1: A Novel Secretory Protein of IGF-Like Family Presents a High Cancer-Promoting Potential! Oncostatin M (OSM): A Member of IL-6 Cytokine Family, An Underestimated Player in Future Targeted Therapies? MAGEA4: a Member of the Melanoma-Associated Antigen (MAGE) Family, a Popular Target for Tumor-Specific Immune Research! IL-6: an Important Participant in the Inflammatory Cascade, One of the Most Functional Cytokines in Various Diseases Research! ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

MAPK signaling pathway mTOR signaling pathway TGF-beta signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Antigen processing and presentation Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications Inflammatory Pain

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.