Sheep Vascular endothelial growth factor A(VEGFA) ELISA kit

Instructions
Code CSB-EL025833SH
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name vascular endothelial growth factor A
Alternative Names RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Abbreviation VEGFA
Uniprot No. P50412
Species Ovis aries (Sheep)
Sample Types serum, plasma
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep VEGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 86-97
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-95
1:8 Average % 97
Range % 91-103
Recovery
The recovery of sheep VEGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 98 91-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.412 2.546 2.479 2.387
2000 1.701 1.748 1.725 1.633
1000 1.032 1.104 1.068 0.976
500 0.658 0.667 0.663 0.571
250 0.364 0.352 0.358 0.266
125 0.287 0.278 0.283 0.191
62.5 0.171 0.177 0.174 0.082
0 0.091 0.092 0.092  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Protein Families PDGF/VEGF growth factor family
Database Links

KEGG: oas:443103

UniGene: Oar.988

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