Bovine Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit

Unavailable
Code CSB-E12780B
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P20959
Lead Time 3-5 working days
Abbreviation IGFBP3
Target Name insulin-like growth factor binding protein 3
Alias tcag7.703, BP-53, IBP3, IGF-binding protein 3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein
Species Bos taurus (Bovine)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Target Details This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
HGNC 5472
RGD 2874
MGI 96438
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %99
Range %96-102
1:200Average %103
Range %101-107
1:400Average %89
Range %85-93
1:800Average %96
Range %93-99
Recovery
The recovery of bovine IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-98
EDTA plasma (n=4)104102-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
500.066 0.064 0.065
250.105 0.111 0.108
12.50.185 0.174 0.180
6.250.352 0.365 0.359
3.120.645 0.686 0.666
1.560.843 0.822 0.833
0.781.004 1.054 1.029
01.534 1.603 1.569
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Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Subcellular Location Secreted
Tissue Specificity Plasma; expressed by most tissues.
Database Links

KEGG: bta:282261

STRING: 9913.ENSBTAP00000047769

UniGene: Bt.64897

Pathway p53 signaling pathway
Cellular senescence

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