guinea pig Immunoglobulin A,IgA ELISA Kit

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Code CSB-E06767Gu
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A,IgA
Species Guinea Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-105
1:2 Average % 90
Range % 87-97
1:4 Average % 97
Range % 92-105
1:8 Average % 90
Range % 85-99
Recovery
The recovery of guinea pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 91-99
EDTA plasma (n=4) 95 89-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
1000 0.364 0.353 0.359
250 0.447 0.456 0.452
125 0.604 0.618 0.611
62.5 0.799 0.813 0.806
15.6 1.136 1.152 1.144
0 1.923 1.894 1.909
References
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