Human Beta-Endorphin,β-EP ELISA Kit

Code CSB-E06821h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Beta-Endorphin,β-EP
Abbreviation
β-EP
Species
Homo sapiens (Human)
Sample Types
serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 99  
Range % 91-110  
1:4 Average % 97  
Range % 92-105  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.537 2.568 2.553 2.443  
2000 2.010 1.999 2.005 1.895  
1000 1.600 1.532 1.566 1.456  
500 1.032 1.122 1.077 0.967  
250 0.567 0.600 0.584 0.474  
125 0.391 0.410 0.401 0.291  
62.5 0.242 0.254 0.248 0.138  
0 0.109 0.111 0.110    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human β-EP ELISA Kit was designed for the quantitative measurement of Human β-EP protein in serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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 Q&A
Q:

I would like to ask about the Human Beta-Endorphin ELISA Kit (CSB-E06821h). In the specification and the manual, I can't understand the difference between the “sensitivity 15.6 pg/mL” and the “detection range of the assay is from 62.5 pg/mL to 4,000 pg/mL”. Is 15.6 pg/mL the minimum detectable level in this ELISA kit? If so, why was the minimum detectable level (15.6 pg/mL) lower than the lowest level of the range (62.5 pg/mL)?

A:

The sensitivity of 15.6pg/ml is the minimum detectable level of this kit, which is obtained by the O.D value of 20 replicates of the zero standard plus their three standard deviations. It is undetectable below this parameter (15.6pg/ml). The measured values ​​of samples in the range of 15.6-62.5pg/ml have large variability. 62.5pg/ml represents the concentration point of the standard S1, and 62.5-4000pg/mL is the detection range of the kit.

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