Human histone deacetylase 3 (HDAC3) elisa kit

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Code CSB-E14413h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O15379
Lead Time 3-5 working days
Abbreviation HDAC3
Protein Biological Process 1 Transcription/Transcription regulation
Target Name histone deacetylase 3
Alias HD3, RPD3, RPD3-2,
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. This protein belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
HGNC 4854
RGD 619977
MGI 1343091
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HDAC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 90-102
1:2 Average % 101
Range % 92-104
1:4 Average % 93
Range % 85-96
1:8 Average % 92
Range % 85-97
Recovery
The recovery of human HDAC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 84-95
EDTA plasma (n=4) 111 97-114
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
100 2.187 2.232 2.210 2.070
50 1.662 1.598 1.630 1.490
25 0.950 0.932 0.941 0.801
12.5 0.563 0.551 0.557 0.417
6.25 0.315 0.327 0.321 0.181
3.12 0.259 0.251 0.255 0.115
1.56 0.192 0.202 0.197 0.057
0 0.142 0.138 0.140  
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Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Subcellular Location Nucleus, Cytoplasm, Cytoplasm, cytosol
Protein Families Histone deacetylase family, HD type 1 subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 4854

OMIM: 605166

KEGG: hsa:8841

STRING: 9606.ENSP00000302967

UniGene: Hs.519632

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