Human procollagen type III ,HPCⅢ ELISA kit

Instructions
Code CSB-E11989h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name procollagen type III ,HPCⅢ
Alternative Names procollagen type 3 ,HPC3,procollagen type III ,HPCⅢ
Abbreviation HPCⅢ
Species Homo sapiens (Human)
Sample Types serum
Detection Range 25 ng/mL-300 ng/mL
Sensitivity 25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 93  
Range % 90-96  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-101  
EDTA plasma (n=4) 97 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
300 1.356 1.342 1.349    
200 0.967 1.023 0.995    
100 0.466 0.482 0.474    
50 0.211 0.199 0.205    
25 0.050 0.048 0.049    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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