human pyridoxal/pyridoxine vitamin B6-kinase,PDXK ELISA Kit

Unavailable
Code CSB-E11765h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O00764
Lead Time 3-5 working days
Abbreviation PDXK
Target Name pyridoxal (pyridoxine, vitamin B6) kinase
Alias C21orf124, C21orf97, DKFZp566A071, FLJ31940, FLJ37311, MGC15873, MGC31754, MGC52346, PKH, PNK, PRED79, pyridoxal kinase|pyridoxamine kinase|pyridoxine kinase|vitamin B6 kinase
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.2 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Target Details This protein phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
HGNC 8819
RGD 621324
MGI 1351869
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PDXK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-98
1:2 Average % 96
Range % 91-100
1:4 Average % 86
Range % 82-90
1:8 Average % 91
Range % 85-96
Recovery
The recovery of human PDXK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 95-105
EDTA plasma (n=4) 89 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.185 2.219 2.202 2.071
1000 1.756 1.811 1.784 1.653
500 1.410 1.343 1.377 1.246
250 0.903 0.975 0.939 0.808
125 0.672 0.684 0.678 0.547
62.5 0.430 0.421 0.426 0.295
31.2 0.275 0.259 0.267 0.136
0 0.132 0.129 0.131  
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Function Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Subcellular Location Cytoplasm
Protein Families Pyridoxine kinase family
Tissue Specificity Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa.
Database Links

HGNC: 8819

OMIM: 179020

KEGG: hsa:8566

STRING: 9606.ENSP00000291565

UniGene: Hs.284491

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