Recombinant Human Interleukin-1 beta(IL1B) (Active)

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Source E.coli
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by its ability to induce NFKB reporter gene expression in HEK 293 cell line is typically 142 pg/mL
Target Names IL1B
Uniprot No. P01584
Research Area Immunology
Alternative Names Interleukin-1 beta; Catabolin; IL1F2; IL1B.
Species Homo sapiens (Human)
Expression Region 117-269aa
Mol. Weight 17.5 kDa
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Gene References into Functions
  1. a genome wide association study for "high" gingival crevicular fluid IL-1beta expression among 4910 European-American adults and identify association signals in the IL37 locus, is reported. PMID: 30206230
  2. Treatment with 30 microg/ml curcumin significantly down-regulated mRNA expression of IL-1beta (p < .05) and protein production of IL-6 (p < .05) in M1 macrophages from Behcet's disease patients but not in M1 macrophage from controls. PMID: 29806793
  3. pp65 mediates human cytomegalovirus immune evasion through downmodulation of IL-1beta. PMID: 30332797
  4. The children in the acute phase of Kawasaki disease had a significantly higher level of IL-1beta than the healthy control group . PMID: 30022755
  5. circSEMA4B serves as a miR-431 sponge to compete with SFRP1 or GSK-3beta for miR-431 binding, thus inhibiting IL-1beta-induced nucleus pulposus degenerative process in intervertebral disc degeneration. PMID: 30251693
  6. IL-1beta-511TT genotype may influence the development of antiretroviral-associated hepatotoxicity and its severity in alcohol and nevirapine users independently and in combination. PMID: 29849489
  7. Impairment in TNF, IL-1beta, and IL-17 production upon stimulation with mycobacterial antigens may contribute to the increased susceptibility to M. tuberculosis infection observed in HTLV-1 infected individuals. PMID: 29523325
  8. rs16944 minor A allele less frequent in type-2 diabetes mellitus PMID: 28634744
  9. This study demonstrated significant differences in the blood levels of IL-1 beta between people with epilepsy and healthy subjects. PMID: 30140987
  10. Polymorphisms of IL-1betab and TNF-a are not a risk of ICC, but an individual with O. viverrini infection has an effect on all genotypes of the TNF-alpha gene that might promote intrahepatic cholangiocarcinoma . Primary prevention of intrahepatic cholangiocarcinoma in high-risk areas is based on efforts to reduce O. viverrini infection. PMID: 30139338
  11. Melatonin inhibits epithelialtomesenchymal transition in gastric cancer cells via attenuation of IL1beta/NFkappaB/MMP2/MMP9 signaling. PMID: 30066836
  12. Study findings suggest that both promoter and exon polymorphisms of IL-1B gene have a significant role in the risk of developing Graves' disease (GD), whereas IL-1RN-VNTR variant has no association with GD in North India Kashmiri population. PMID: 29454070
  13. The IL-6 expression was consistently associated with necrotizing fasciiitis severity, whereas IL1B had the strongest association with 30-day mortality. PMID: 28176831
  14. Taken together, the silencing of H4R inhibited the H4R mediated Mast cell functions and SAPK/JNK phosphorylation. Furthermore, the H4R activation utilized SAPK/JNK signaling pathway for IL-1beta release in HMC-1 cells PMID: 29863427
  15. IL-1beta/IL-6 network is highly expressed in the colorectal cancer (CRC) microenvironment, indicating that this network is important in the progression of CRC PMID: 29803656
  16. Colombian individuals with high African ancestry proportions at locus 2q14 harbour more IL1B-CGTC copies and are consequently at an increased risk of colorectal cancer. PMID: 28157220
  17. Study found a statistically significant association between the IL-1B rs16944 polymorphism and febrile seizures (FS) risk in Caucasians and Asians. The TT+CT genotypes were associated with a 1.43 times increased risk for FS in Caucasians, while an increased risk of 1.39 times was observed for FS development for the TT homozygotes in Asians. PMID: 29808330
  18. Preincubation of LAD2 cells with the natural flavonoid methoxyluteolin (1-100 mM) inhibits (P < 0.0001) secretion and gene expression of IL-1beta, procaspase-1, and pro-IL-1beta. Mast cell secretion of IL-1beta in response to SP and IL-33 reveals targets for the development of antiinflammatory therapies. PMID: 30232261
  19. in Caco-2 CFTR-shRNA cells, the EGFR ligand EREG is overexpressed due to an active IL-1beta autocrine loop that indirectly activates EGFR, constituting new signaling effectors for the CFTR signaling pathway, downstream of CFTR, Cl(-) , and IL-1beta. PMID: 29091309
  20. Caspase-1-dependent IL-1beta processing and secretion require the AIM2 inflammasome pathway in human dental pulp cells and the AIM2 inflammasome pathway is critical for regulation of the dental pulp immune response. PMID: 29178062
  21. Genetic Polymorphisms of IL1B, IL6, and TNFalpha in a Chinese Han Population with Pulmonary Tuberculosis. PMID: 29888256
  22. Our results demonstrate that in the early stage of sepsis, JMJD3 contributes to high levels of neutrophil mPR3 expression and thereby to the production of the inflammatory cytokine IL-1beta PMID: 29621735
  23. In the current study, we found a clear inflammagenesis gene signature in COPD small airway epithelium, with increased gene expression of CCL2, CCL7, IL1B and IL1R2 in lung small airway epithelial cells. We provide evidence for IL1B as a novel player in inflammation. PMID: 29616282
  24. The results of this study suggested that rs16944 of IL-1 beta may act as a risk factor for multiple system atrophy. PMID: 29251119
  25. IL-1 role in cardiovascular diseases [review] PMID: 29549570
  26. Sendai Virus V Protein Inhibits the Secretion of Interleukin-1beta by Preventing NLRP3 Inflammasome Assembly. PMID: 30021903
  27. This study suggested that -511 C/T (rs16944) and -31 C/T (rs1143627) polymorphisms in IL-1beta gene may not be involved in RPL in Iranian Azeri women. Also the promoter polymorphism of the IL-1RN gene may not play a role in the susceptibility to RPL. PMID: 29281611
  28. IL-1beta and CTHRC1 are upregulated in patients with Osteoarthritis. PMID: 29393342
  29. our observations indicated that IL-1beta-mediated NF-kappaB signaling augments the osteosarcoma cell growth via modulating MiR-376c/TGFA axis PMID: 29441940
  30. Insulin resistance correlates positively with IL-1beta among non-diabetic hemodialysis patients. PMID: 29436520
  31. The baseline concentrations of IL-6 and IL-1beta were significantly higher in the Major Depressive Disorder group than those in the control group. There was no significant difference of TNF-alpha between the two groups. PMID: 29587166
  32. The genetic association between the studied SNPs of the IL-1beta gene and recurrent apththous stomatitis remains controversial and requires further investigation. PMID: 28942153
  33. The rs16944 TT genotype of Il-1 beta is associated with mesial temporal lobe epilepsy with hippocampal sclerosis development what may be explained by the higher IL-1beta levels produced by this genotype. PMID: 28675059
  34. Our meta-analysis proved that IL-1beta + 3954C/T is associated with MI susceptibility, especially among Caucasian populations. PMID: 30045312
  35. In this longitudinally monitored male population, observed effect of baseline central adiposity on future periodontitis progression is conditional on proinflammatory IL-1 genetic variations. PMID: 27541081
  36. In Pakistani population, an increased risk of gastric cancer development is associated with the carriage of IL-1B-511*T and IL-1RN*2 alleles PMID: 28110439
  37. This study revealed that the P2X7R/NLRP3 pathway plays important roles in IL-1beta secretion and inhibition of Toxoplasma gondii proliferation in small intestinal epithelial cells. PMID: 29291748
  38. These results indicate that tumorderived IL1beta enhanced stromal glycolysis and induced oneway lactate flow from the tumor mesenchyme to transformed epithelium, which promotes oral squamous cell carcinoma cell proliferation PMID: 29207019
  39. Therefore, the common allergen Der f1 was not only found to induce allergy, but also led to pyroptosis and IL1beta secretion via the NLRP3caspase1 inflammasome in human bronchial epithelial cells PMID: 29207030
  40. Increased production of IL-1beta in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590
  41. IL-1beta is involved in the regulation of OPN levels during respiratory syncytial virus infection. PMID: 29677209
  42. Sequencing results of samples from different individuals discovered that polymorphisms on the loci 154 and 486 significantly increased the risks of obstructive sleep apnea syndrome and affected IL-1beta protein expression. However, we did not conduct in-depth research PMID: 28742193
  43. sauchinone inhibited inflammatory response in IL-1beta-stimulated human chondrocytes probably through inhibiting the activation of NF-kappaB signaling pathway. These data suggest that sauchinone may be a potential agent in the treatment of OA. PMID: 29356224
  44. VPS4B might facilitate chondrocyte apoptosis in Osteoarthritis via p38 MAPK signaling pathway. PMID: 28744712
  45. this study shows that IL-1beta limits the level of NK cell activation by inducing apoptosis and suppression as a homeostatic regulatory function PMID: 27086951
  46. Higher IL-1beta levels are associated with post-stroke depression at 2 weeks in the presence of the -511T allele with a borderline significant interaction term and with any +3953C/T polymorphism without a significant interaction term. PMID: 28844626
  47. In multivariate analysis, the IL1B rs16944 TT and TNF rs1799964 TC genotypes were significantly associated with intrauterine cytomegalovirus infection. Twenty-two out of 72 congenitally infected newborns had confirmed sensorineural hearing loss. Carriers of CT or TT genotype of CCL2 rs13900 had increased risk of hearing loss at birth and at 6 months of age. PMID: 28501927
  48. TNFalpha and IL-1beta mediated expression of E-selectin preferentially attract sialyl Lewis(X) mediated mucosal-associated invariant T cell migration into the synovial fluid of rheumatoid arthritis patients. PMID: 28756336
  49. These data indicate a process of NF-kappaB-induced miR-506 suppression and JAG1 upregulation upon IL-1beta induction. PMID: 28926924
  50. Effective IL1beta and CCL2 antagonists are currently in clinical review to treat benign inflammatory disease, and their transition to the cancer clinic could have a rapid impact PMID: 28790030
  51. SNPs associated with recurrent aphthous stomatitis in a Polish cohort PMID: 27935125
  52. findings demonstrate that post-transcriptional regulation via p38 MAPK plays a central role in the rapid synthesis of pro-IL-1beta in response to MSU crystals, which is an essential step for IL-1beta production in human monocytes. PMID: 27694988
  53. our results indicated that IL-1beta treatment resulted in a significant increase in expression of the transcriptional factor interferon regulatory factor-1 (IRF-1) at both the mRNA and protein levels, which was significantly ameliorated by treatment with Nebivolol. The combination of these findings suggests that Nebivolol can potentially be applied in human osteoarthritis treatment PMID: 28512729
  54. The rs1143634 polymorphism was associated with elevated risk of chronic periodontitis. PMID: 29783069
  55. Decreased invasion caused by AKR1C1 knockdown suggests a novel role of AKR1C1 in cancer invasion, which is probably due to the regulation of Rac1, Src, or Akt. An inflammatory cytokine, interleukin-1beta, was found to increase AKR1C1 in bladder cancer cell lines. PMID: 27698389
  56. No significant difference was seen in mRNA levels among different promoter genotype for IL1B in SCA3 patients vs. controls, except a previously reported higher level in those with the IL1B*T allele. These patients also showed an earlier age of onset than those homozygous for IL1B*C. PMID: 27246313
  57. A strong association between cytokine TNF-alpha, IL-6 and IL-1beta gene promoter polymorphisms and modulation of transcript levels with susceptibility to nephropathy in diabetes subjects. PMID: 29605608
  58. Under specific experimental conditions, secretion of IL-1beta and FGF2 is triggered by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent formation of pores across the plasma membrane. PMID: 28871048
  59. We found that three IL-1beta polymorphisms rs1143634, rs1143633, and rs1143643 were associated with allergic asthma risk (P = 0.034; OR = 1.523; P = 0.024, OR = 1.477; 0.044, OR = 1.420, respectively).We then analyzed if IL-1beta expression was altered in serum and we found that asthmatic children showed significantly higher IL-1beta levels than healthy controls (P = 0.047). PMID: 29034996
  60. The interleukin 1 beta (IL1B)-511C/T polymorphism is found to be associated with a significant increased risk for coronary heart disease (CHD), and the interleukin 1 receptor antagonist protein (IL1RN)+8006T/C polymorphism may play a protective role in CHD. PMID: 29169428
  61. Data show that infecting unencapsulated E. faecalis cps2 is a stronger stimulator for toll like receptor 2 (TLR2) and interleukin-1beta (IL-1beta) mRNAs, but not for inducible nitric oxide synthase (iNOS) mRNA. PMID: 28800779
  62. Post-transcriptional regulation of COX-2 mRNAs translation by SGs indicates a role in IL-1beta-mediated catabolic response that could be therapeutically targeted in Osteoarthritis. PMID: 27271770
  63. Our findings revealed that I3C inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1beta) were overexpressed upon I3C treatment PMID: 28812970
  64. This study investigated if there were structural differences between hematomas from normal and delayed healing bone defects and whether such differences were linked to changes in the expression of IL-1beta. PMID: 27767056
  65. glioma cells expressing CD133 are capable of modulating tumor microenvironment through the IL-1beta signaling pathway. PMID: 28736425
  66. This study demonstrated that IL1B expression levels are related to major depressive disorder and conjunctly mediate the effect of childhood maltreatment history on the risk of developing major depressive disorder. PMID: 28384542
  67. GDNF has a role in lower than expected motor development, but while IL-1beta and CXCL8/IL-8 values were higher in the group with typical motor development among preterm neonates PMID: 28553016
  68. Our findings call into question the role of IL-1beta in the diabetic pancreas as it has been proposed in previous literature. Additionally, our results regarding the localization of IL-1beta should lead to further investigation into the role of IL-1beta in the physiology of pancreatic alpha cells. PMID: 28325643
  69. IL-1beta rs1143627 polymorphism is not related to periodontal disease susceptibility in the overall population based on the current evidence. PMID: 28404906
  70. Our results do not show any evidence of association between COPD and IL-1beta -511 and +3954 gene polymorphisms in Turkish population PMID: 28990887
  71. Our findings suggest that AhR-mediated IL-1beta regulation in cerebral endothelium could induce BBB breakdown and contribute to the pathogenesis of a variety of neurologic disorders. PMID: 28285151
  72. the IL1B -511 T > C polymorphism may serve as important risk factor for recurrent miscarriage while the IL6 -634C > G polymorphism may protect against the risk of recurrent miscarriage PMID: 29017513
  73. Our data suggests that the polymorphism in genes IL-1beta, IL-1Ra and FABP1 included in this study are not associated with PCOS development, but can influence important biochemical and metabolic parameters in PCOS. PMID: 28405733
  74. both RAGE and mitochondrial damage primed NLRP3 and pro-IL-1beta activation as upstream signals of NF-kappaB activity, whereas mitochondrial damage was critical for the assembly of inflammasome components. These results revealed that accumulation of AGEs in NP tissue may initiate inflammation-related degeneration of the intervertebral disc via activation of the NLRP3 inflammasome. PMID: 28224704
  75. Here, the authors used a set of isogenic streptococcal NAD(+)-glycohydrolase mutants and a macrophage infection model and report that streptococcal streptococcal NAD(+)-glycohydrolase inhibits the innate immune response by decreasing inflammasome-dependent interleukin 1beta (IL-1beta) release from infected macrophages. PMID: 28720729
  76. A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
  77. Elevated serum IL-1beta and IL-17 levels may be used as a biomarker for indicating persistent neutrophilic airway inflammation and potential ongoing exacerbation of COPD. PMID: 28490868
  78. The genotypic polymorphisms of IL-1beta -511 and +3954, and of MUC7 VNTR, had no direct associations with the development of BMS. However, the T allele of IL-1beta -511 may increase the risk of BMS by increasing psychological asthenia. PMID: 27246755
  79. serum levels of IL-1beta were able to prognostically stratify our cohort of iDCM patients. The precise cut-off of IL-1beta associated with bad outcome in iDCM remains to be determined in larger, independent cohorts of patients. PMID: 28212578
  80. Biological complications in implant-supported overdentures were found to be mostly associated with the presence of allele 2 of the IL1B gene. PMID: 28223139
  81. The addition of vildagliptin to metformin treatment in patients with type 2 diabetes and coronary artery disease led to a significant suppression of the IL-1ss elevation. PMID: 28532406
  82. High interleukin 1 beta expression is associated with neuroinflammation. PMID: 26549801
  83. Data show that cyclic stretch activated the nucleotide-binding oligomerization domain-like receptor containing pyrin domain 1 and 3 (NLRP1 and NLRP3) inflammasomes and induced the release of IL-1beta and pyroptosis via a caspase-1-related mechanism in human periodontal ligament cells (HPDLCs). PMID: 27626170
  84. Our findings suggest that there is a prevalence of mutated allele of IL-1 gene cluster in our population, which may be responsible for renal dysfunction. PMID: 28359253
  85. Demonstrate that RARalpha was frequently elevated in gastric carcinoma and exerted oncogenic properties via positive feedback loop of IL-1beta/Akt/RARalpha/Akt signaling. PMID: 28035062
  86. Icariin inhibits MMP1, MMP3 and MMP13 expression through MAPK pathways in IL1betastimulated SW1353 chondrosarcoma cells PMID: 28447732
  87. Sesamin showed anti-inflammatory effects in IL-1beta-stimulated chondrocytes by activating Nrf2 signaling pathway. PMID: 27863411
  88. Data showed that IL-1beta downregulates 15-PGDH expression in pancreatic ductal adenocarcinoma (PDAC) cells, and that IL-1beta expression was inversely correlated with 15-PGDH levels in frozen PDAC tissues resulting in poor prognosis. PMID: 29224225
  89. Concentration of IL-1beta was significantly higher in patients with heart failure (HF) compared with non-HF and control groups. Results of the distribution of IL-1beta-31T/C genotypes and allele frequencies did not show any significant difference between the three groups. Serum levels of IL-1beta were found to be higher among TT genotype than TC and CC genotype. PMID: 29182005
  90. the activation of Rho GTPases by the CNF1 toxin during E. coli-triggered bacteremia leads to a GR1(+)cell-mediated efficient bacterial clearing and improves host survival. Host alarm requires the Caspase-1/IL-1beta signaling axis. PMID: 26492464
  91. The level of miR-216b was significantly higher and Smad3 expression was obviously lower in osteoarthritis cartilage and IL-1beta-induced chondrocytes than in normal tissues and cells. PMID: 28356485
  92. C/EBPbeta overexpression or knockdown did not change the levels of IL-1beta-induced SOCS1. SOCS1 regulated the levels of C/EBPbeta mRNA by ubiquitination of C/EBPbeta as well as transcriptional regulation in human chondrocytes. PMID: 27339399
  93. The obtained data demonstrate that the high level of production of IL-1b and IL-4 in GN patients causes hypothyroidism resulting in the formation of nephrotic syndrome. PMID: 29244911
  94. An association with IL-1beta-511 locus and IL-1beta-511-TLR4-896 diplotype (CC-AA) and Type 2 Diabetes Mellitus is discussed. PMID: 28620993
  95. IL-1beta degrades the ventilatory hypoxic response by stimulating production of prostaglandin PMID: 28177673
  96. The plasma levels of interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, tissue inhibitor of metalloproteinases (TIMP)-1 are increased in myelofibrosis (MF) patients. PMID: 27304059
  97. Absolute numbers of MIF and IL-1beta mRNA molecules are both accurate and reliable predictors of antidepressant response. PMID: 27207917
  98. results showed that Gypenoside dose-dependently inhibited IL-1beta-induced NO and PGE2 production in human osteoarthritis chondrocytes. PMID: 28422402
  99. tuberculous lymphadenitis (TBL) is therefore, characterized by reduced systemic and antigen-specific concentrations of IL-1beta and IL-18, which are reversible following anti-TB treatment, indicating that these cytokines are potential correlates of protective immunity in TBL. PMID: 27794266
  100. p27(kip1) overexpression regulates IL-1beta in the microenvironment of stem cells and eutopic endometriosis PMID: 26817396

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Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Secreted
Protein Families IL-1 family
Tissue Specificity Expressed in activated monocytes/macrophages (at protein level).
Database Links

HGNC: 5992

OMIM: 147720

KEGG: hsa:3553

STRING: 9606.ENSP00000263341

UniGene: Hs.126256

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