Code | CSB-MA878942A0m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:1000-1:5000 |
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
The PD-L1 monoclonal antibody was harvested from the hybridomas, which were formed by fusing the spleen cells and myeloma cells. The spleen cells were isolated from the mouse immunized with recombinant human PD-L1 protein (19-238aa). The resulting PD-L1 monoclonal antibody is highly specific for human PD-L1 protein and can be used in a variety of research applications, including ELISA, WB, IHC, IF, and FC. Protein G purification of this antibody brought its purity up to 95%.
PD-L1 mainly regulates the immune system by interacting with its receptor PD-1, which is found on activated T cells. The binding of PD-L1 to PD-1 leads to the inhibition of T-cell activity, preventing excessive immune responses that can result in tissue damage and autoimmune disorders. PD-L1 is also exploited by cancer cells as a mechanism of immune evasion, allowing them to evade attack by the immune system.
Applications : WB
Sample type: Human Cell
Sample dilution: 1:1000
Review: The expression of PD-L1 protein was determined by Western blot. They found that down-regulation of down regulation of PD-L1 at the protein level is observed in OV3R-PTX cells compared to OVCAR-3 cells.
By Anonymous