Recombinant Macaca fascicularis Angiotensin-converting enzyme(ACE2),partial (Active)

In Stock
Code CSB-MP3414MOV
Size $850
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml. Biological Activity Assay
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Product Details

Description

CUSABIO cloned the coding sequence of Macaca fascicularis angiotensin-converting enzyme (ACE2) (amino acids 18-741) to produce a fusion recombinant protein with the Fc portion of the human IgG1 tag in mammalian cells. This recombinant Macaca fascicularis ACE2 protein is an active protein, whose bioactivity has been measured by its binding with SARS-CoV-2-S1-RBD (EC50 is 5.638-9.496 ng/ml). It is characterized by high purity (>94%) and low endotoxin (<1.0 EU/ug protein). Due to the glycosylation, it has an apparent molecular mass of 114 kDa on the gel. It is in stock now.

High expression of ACE2 is restricted to the endothelial cells of the arteries, arterioles, and venules of the heart and kidney. ACE2 is involved in regulating blood pressure and keeping blood pressure homeostasis. ACE2 binding to the SARS-CoV-2 S protein facilitates the viral entry into host cells, resulting in the lethal viral infection.

Purity Greater than 94% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml.
Target Names ACE2
Uniprot No. A0A2K5X283
Research Area Cancer
Molecular Characterization
Species Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Source Mammalian cell
Expression Region 18-741aa
Target Protein Sequence QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYKDYGDYWRGNYEVNGVDGYDYNRDQLIEDVERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLLNMLKLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRTYFLEIKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRISRSRINDAFRLNDNSLEFLGIQTTLAPPYQSPVTT
Mol. Weight 112.7 kDa
Protein Length Partial
Tag Info C-terminal hFc-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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