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Product Name:

HIST1H4A (Ab-5) Antibody

Code : CSB-PA010429OA05nbutHU
Size : US$319
Uniprot NO. : P62805
Abbreviation : Histone H4
Storage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image :
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
    All lanes: HIST1H4A antibody at 0.08ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • IHC image of CSB-PA010429OA05nbutHU diluted at 1:10 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA010429OA05nbutHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen : Peptide sequence around site of butyrly-Lys (5) derived from human Histone H4
Raised in : Rabbit
Species Reactivity : Human, Mouse
Tested Applications : ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:1-1:100, IF:1:50-1:200
Relevance : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form : Liquid
Conjugate : Non-conjugated
Storage Buffer : Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification method : Antigen Affinity Purified
Isotype : IgG
Clonality : Polyclonal
Alias : Histone H4, HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4, H4/A H4FA; H4/I H4FI; H4/G H4FG; H4/B H4FB; H4/J H4FJ; H4/C H4FC; H4/H H4FH; H4/M H4FM; H4/E H4FE; H4/D H4FD; H4/K H4FK; H4/N H4F2 H4FN HIST2H4; H4/O H4FO;
Species : Homo sapiens (Human)
Research Area : Epigenetics and Nuclear Signaling
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