Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: HIST1H4A antibody at 0.6ug/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
IHC image of CSB-PA010429OA05npropHU diluted at 1:5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA010429OA05npropHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating HIST1H4A in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010429OA05npropHU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429OA05npropHU(5ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)
Peptide sequence around site of propionyl-Lys (5) derived from human Histone H4
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.