RFC1 Antibody

Code CSB-PA019588KA01HU
Size US$398
  • Western blot analysis of extracts of MCF-7 cell lines, using RFC1 antibody.
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Product Details

Uniprot No. P35251
Target Names RFC1
Alternative Names A1 140 kDa subunit antibody; A1 antibody; A1 P145 Activator 1 large subunit antibody; Activator 1 140 kDa subunit antibody; Activator 1 large subunit antibody; Activator 1 subunit 1 antibody; DNA binding Protein PO GA antibody; DNA-binding protein PO-GA antibody; MHC binding factor beta antibody; MHCBFB antibody; PO GA antibody; RECC1 antibody; Replication factor C (activator 1) 1, 145kDa antibody; Replication factor C 140 kDa subunit antibody; Replication factor C antibody; Replication factor C large subunit antibody; Replication factor C subunit 1 antibody; Replication factor C1 antibody; RF-C 140 kDa subunit antibody; RFC antibody; RFC1 antibody; RFC1_HUMAN antibody; RFC140 antibody; RFC140 Replication Factor C 140 kDa subunit antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Recombinant protein of Human RFC1
Immunogen Species Homo sapiens (Human)
Isotype IgG
Purification Method Affinity purification
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Store at -20°C or -80°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Gene References into Functions
  1. Human CTF18-RFC clamp-loader complexed with non-synthesising POLE efficiently loads the PCNA sliding clamp. PMID: 28199690
  2. RFC depletion had a negative impact on LANA's ability to replicate and maintain viral DNA in cells containing artificial KSHV episomes or in infected cells, leading to loss of virus. PMID: 25071216
  3. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
  4. report that the large subunit (RFC1) of replication factor C is cleaved in Hutchinson-Gilford progeria syndrome cells, leading to the production of a truncated RFC1 of ~ 75 kDa PMID: 22168243
  5. We identified a variant in each of 3 genes (RFC1, SCN1A, ANPEP) potentially implicated in susceptibility to severe neurological complications in West Nile Virus disease. PMID: 21881118
  6. Small interfering RNA-mediated knockdown of RFC1 impairs upstream removal of UV lesions and abrogates the downstream recruitment of DNA polymerase delta. PMID: 20713449
  7. analysis of the DNA-bound BRCA1 C-terminal region structure from human replication factor C p140 and model of the protein-DNA complex PMID: 20081198
  8. interaction with histone deacetylase HDAC1 PMID: 12045192
  9. RFC (p140) has an important role as a regulator of transcription and NF-kappaB activity PMID: 12509469
  10. destabilization of the RF-C complex by CDKs may inactivate the RF-C complex at the end of S phase PMID: 12947101
  11. NMR resonance assignments of the BRCT region of the large subunit PMID: 15772763
  12. Ctf18-RFC interacts physically with pol eta, which allows DNA replication forks to overcome interference by various template structures, including damaged DNA and DNA-protein complexes that maintain chromosome cohesion PMID: 17545166
  13. Data show that replication factor C (RFC), via several stimulatory motifs per molecule, potently activates flap endonuclease 1 (FEN1), making RFC a critical partner with FEN1 for the processing of eukaryotic Okazaki fragments. PMID: 19208620
  14. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468

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Subcellular Location Nucleus
Protein Families Activator 1 large subunit family
Tissue Specificity Wide tissue distribution. Undetectable in placental tissue.
Database Links

HGNC: 9969

OMIM: 102579

KEGG: hsa:5981

STRING: 9606.ENSP00000371321

UniGene: Hs.507475


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