TNF Antibody

Code CSB-PA004963
Size US$167
  • Western Blot analysis of mouse liver cells using TNF-α Polyclonal Antibody
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Product Details

Uniprot No. P01375
Target Names TNF
Alternative Names APC1 antibody; APC1 protein antibody; Cachectin antibody; DIF antibody; Differentiation inducing factor antibody; Macrophage cytotoxic factor antibody; Tnf antibody; TNF superfamily member 2 antibody; TNF superfamily, member 2 antibody; TNF, macrophage derived antibody; TNF, monocyte derived antibody; TNF-a antibody; TNF-alpha antibody; TNFA antibody; TNFA_HUMAN antibody; TNFSF2 antibody; Tumor necrosis factor (TNF superfamily member 2) antibody; Tumor necrosis factor alpha antibody; Tumor necrosis factor antibody; Tumor necrosis factor ligand superfamily member 2 antibody; Tumor Necrosis Factor, Membrane Form antibody; Tumor necrosis factor, soluble form antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Synthesized peptide derived from the Internal region of Human TNF-α.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:20000
Protocols Western Blotting(WB) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective
Gene References into Functions
  1. Genetic predisposition to rheumatoid arthritis in Russian population in the Republic of Karelia is associated with the presence of the GG TNF-alpha genotype. PMID: 30225702
  2. Treatment with 30 microg/ml curcumin also significantly diminishes the protein production of TNFalpha in Behcet's disease patients (p < .01) and healthy controls (p < .05) M1 macrophages. PMID: 29806793
  3. the protective role of the A allele in TNF-alpha 238A/G but not TNF-alpha 308A/G against the occurrence of juvenile idiopathic arthritis in the Caucasian population (Meta-Analysis) PMID: 30412082
  4. TNF-alpha expressed regionally in Epicardial Adipose Tissue may exert potent effects on the progression of coronary atherosclerosis PMID: 28931782
  5. The effect of the pro-inflammatory diet on concentrations of TNF-alpha was more pronounced in pregnant women reporting higher levels of stress. PMID: 30200631
  6. TNF-alpha -308G/A polymorphism is not associated with the risk to develop of bullous pemphigoid and alopecia areata in our Iranian cohort PMID: 29843231
  7. data support that only the promoter single-nucleotide polymorphism (SNP) rs1800629 within the TNF-alpha gene is associated with increased risk for developing Graves' disease (GD), especially in European population. Future large-scale studies are required to validate the associations between TNF-alpha gene and GD. PMID: 29440561
  8. We studied 173 polymorphisms to establish an association with the response to anti-TNF drugs in patients with moderate-to-severe plaque psoriasis (N=144). PMID: 27670765
  9. The SNP rs4819554 in the promoter region of IL17RA significantly influences the response to anti-TNF drugs at week 12. PMID: 27670766
  10. TNF-alpha promoter gene polymorphisms and/or haplotypes are risk factors of Nephrotic syndrome and resistance to steroid among Egyptian children PMID: 28803697
  11. The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for nasal polyposis in the north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. PMID: 30003390
  12. results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
  13. The single nucleotide polymorphisms rs361525, rs1800629, and rs17999645 of tumor necrosis factor-alpha were significantly correlated with the diagnosis of cervical cancer PMID: 29940817
  14. Rs11541076 in IRAK3, a negative regulator of TLR signalling, as a predictor of anti-TNF treatment response. PMID: 27698401
  15. In this Brazilian population, TNF and IL17 gene polymorphisms responsible for the expression of important inflammatory cytokines were associated with overall spondyloarthritis and, specifically, with ankylosing spondylitis and psoriatic arthritis, regardless of gender and HLA-B27 PMID: 29849482
  16. by restraining TNFR1 at the cell surface via sialylation, ST6Gal-I acts as a functional switch to divert signaling toward survival. These collective findings point to a novel glycosylation-dependent mechanism that regulates the cellular response to TNF and may promote cancer cell survival within TNF-rich tumor microenvironments. PMID: 29233887
  17. The TNF*A allele confer susceptibility to AIH in the Tunisian patients and is associated with increased production of TNF-alpha. Anti-TNF antibodies could be an alternative to the use of corticotherapy and may avoid the exacerbated immune response in Autoimmune hepatitis. PMID: 29845365
  18. Impairment in TNF, IL-1beta, and IL-17 production upon stimulation with mycobacterial antigens may contribute to the increased susceptibility to M. tuberculosis infection observed in HTLV-1 infected individuals. PMID: 29523325
  19. LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID: 30233161
  20. TNF-alpha-308G>A polymorphism affects the overall survival of cancer patients and is a potential therapeutic target for cancer. PMID: 30407345
  21. Many inflammatory pathologies are now recognized to be driven by aberrant TNF-induced cell death, which, in most circumstances, depends on the kinase Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). [review] PMID: 29217118
  22. High TNF-alpha expression is associated with retinopathy of prematurity. PMID: 29274846
  23. Tumour necrosis factor-alpha selectively reduces BMPR-II transcription and mediates post-translational BMPR-II cleavage via the sheddases, ADAM10 and ADAM17 in pulmonary artery smooth muscle cells. PMID: 28084316
  24. Polymorphisms of IL-1betab and TNF-a are not a risk of ICC, but an individual with O. viverrini infection has an effect on all genotypes of the TNF-alpha gene that might promote intrahepatic cholangiocarcinoma . Primary prevention of intrahepatic cholangiocarcinoma in high-risk areas is based on efforts to reduce O. viverrini infection. PMID: 30139338
  25. In placenta, when gestational age was controlled for, partial correlation revealed a significant positive correlation between TNF-alpha and MMP-9 only in the second trimester. PMID: 28820024
  26. Study shows that in human endometrial stromal cells, high tumor necrosis factor levels negatively affect the insulin action through decreased adiponectin signaling and glucose transporter type 4 protein. This could explain the failures observed in endometrial function of obese women with polycystic ovary syndrome. PMID: 28946816
  27. Three single nucleotide polymorphisms (SNPs) within P2X4R and two SNPs within CAMKK2 influenced concentrations of TNFalpha in peripheral blood mononuclear cells, but these SNP did not associate with risk for HIV-associated sensory neuropathy in South Africans. PMID: 29428485
  28. Results indicated that the proinflammatory cytokine TNF-alpha impairs endothelial tight junctions and promotes monocyte-endothelial cell adhesion by upregulating beta-site amyloid precursor protein enzyme 1 expression through activating PKC signaling and sequentially cleaving alpha-2, 6-sialic acid transferase 1. PMID: 28091531
  29. These findings reveal that PrP enhances the responses to TNF-alpha, promoting proinflammatory cytokine production, which may contribute to inflammation and tumorigenesis. PMID: 28900035
  30. This study found that the protein and mRNA expression levels of the cytokines TNF-alpha is significantly increased. PMID: 28476335
  31. Taking together, these results suggest that Wnt/beta-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-alpha-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. PMID: 28112190
  32. Data suggest that, in children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio. PMID: 28749076
  33. TNFalpha differently regulated the levels of PPARalpha, LXRalpha, and LXRbeta binding to the apoA-I gene promoter in THP-1 cells. Obtained results suggest a novel tissue-specific mechanism of the TNFalpha-mediated regulation of apoA-I gene in monocytes and macrophages and show that endogenous ApoA-I might be positively regulated in macrophage during inflammation. PMID: 29442267
  34. this is the first evidence to suggest that TET2 mutations promote clonal dominance with aging by conferring TNFalpha resistance to sensitive bone marrow progenitors while also propagating such an inflammatory environment. PMID: 29195897
  35. Anti-rotavirus effect of TNF-alpha was achieved by NFkappaB-regulated genes via the activation of classical nuclear factor kappaB (NF-kappaB) signaling. PMID: 29859235
  36. Results revealed that heterozygous genotype GA of TNF-alpha-238 (rs 361525) SNP significantly increased the risk of adverse-outcome (mortality rate), regardless of organ dysfunction or severity of sepsis. PMID: 29978383
  37. in the patients with primary depression, depressive symptoms were associated with TNF-alpha PMID: 30148175
  38. Addition of TNFalpha to podocytes causes CD80 upregulation, actin reorganization and podocyte injury. PMID: 29022109
  39. The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID: 30280549
  40. TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
  41. the results of the present study suggested that overexpression of KLF15 in Eahy926 cells exhibited a protective effect against TNFalpha induced dysfunction via activation of Nrf2 signaling and inhibition of nuclear factor kappaB signaling. PMID: 29956764
  42. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
  43. Elevated A20 promotes TNF-induced and RIPK1-dependent intestinal epithelial cell death PMID: 30209212
  44. TNF-alpha GG genotype at -238 and GG haplotype at positions -308 and -238 were associated with Kawasaki disease in an Iranian population. PMID: 27455075
  45. We have shown that a TNFalpha gene polymorphism, rs1800629, is highly significantly associated with postmenopausal osteoporosis and BMD in the female Han Chinese population PMID: 29481288
  46. allele -308 A TNF-alpha may have a role in progression of rheumatoid arthritis in a North Indian population PMID: 28748515
  47. ATF3 mediates the inhibitory action of TNF-alpha on osteoblast differentiation and that the TNF-alpha-activated JNK pathway is responsible for the induction of Atf3 expression. PMID: 29605296
  48. TNF-alpha, DKK1, and OPG have roles in pathogenesis of knee osteoarthritis PMID: 28676900
  49. Btk acts in the TLR7/8 pathway and mediates Ser-536 phosphorylation of p65 RelA and subsequent nuclear entry in primary human macrophages. PMID: 29567473
  50. Results indicated that prolonged tumor necrosis factor (TNFalpha) exposure could have detrimental consequences to endothelial cells by causing senescence and, therefore, chronically increased TNFalpha levels might possibly contribute to the pathology of chronic inflammatory diseases by driving premature endothelial senescence. PMID: 28045034
  51. Serum TNF-alpha levels are elevated in women with clinically symptomatic uterine fibroids. PMID: 29809057
  52. When comparing pulmonary tuberculosis patients with latent TB infection subjects, significant associations with disease development were observed for SNPs of IL6 and TNFalpha. PMID: 29888256
  53. SNPs in TNF are related to ankylosing spondylitis susceptibility in a Chinese Han population. PMID: 29230494
  54. this study evaluated TNF -1031T/C (rs1799964), -376G/A (rs1800750), -308G/A (rs1800629) -238G/A (rs361525) polymorphisms association with RA susceptibility in a sample of Mexican patients. Our results suggest that the TNF -1031T/C, -376G/A, -308G/A, -238G/A, are not associated with RA susceptibility in a sample of Mexican patients. PMID: 29404828
  55. NF-alpha production by degenerated nucleus pulposus cells was downregulated by adiponectin administration in a dose- and time-dependent manner PMID: 28767622
  56. These studies show that TNF-alpha stimulates human FDC-SP gene transcription by targeting YY1, GATA, C/EBP2 and C/EBP3 in the FDC-SP gene promoter. PMID: 29356241
  58. The results of this study suggested that rs1799964 of TNF-alpha may act as a risk factor for multiple system atrophy. PMID: 29251119
  59. findings of present study suggest that the TNF-alpha/IL-10 ratio may play a vital role in the development of coronary artery disease of North Indian population PMID: 28988596
  60. TNF-alpha stimulates AMTN gene transcription in human gingival epithelial cells via C/EBP1, C/EBP2, and YY1 elements in the human AMTN gene promoter. PMID: 29282478
  61. polymorphisms in TNF-alpha gene may play a role in mediating the risk to disease severity of Severe fever with thrombocytopenia syndrome in Chinese Han population PMID: 29939989
  62. the relationship between TNF-alpha -1031T/C polymorphism and plasma level of TNF-alpha; the role of TNF-alpha -1031T/C in cancer cachexia PMID: 29802455
  63. Data suggest that serum levels of TNFA, LH (luteinizing hormone), and androstenedione are reliable predictors/biomarkers of spontaneous ovulation following laparoscopic ovarian drilling in recruiting surgical candidates with CCR-PCOD (clomiphene citrate resistance polycystic ovarian disease). This study was conducted in Egypt. PMID: 29408743
  64. TNF-alpha-induced expression of transport protein genes in HUVEC cells is associated with enhanced expression of RELB and NFKB2. PMID: 29658079
  65. these results suggest a minimal effect of the -308 and -238 TNF-alpha gene polymorphisms in dengue patients and that their increased serum levels of TNF-alpha are independent of genotypes. PMID: 28650708
  66. The rs1800629 A-allele in the TNF gene was associated with an increased risk of dengue hemorrhagic fever (OR = 3.4, CI = 1.235-9.284 p = 0.0212) whereas SNPs rs4804803, rs2780831, rs1801274, rs231775, rs12979860, and rs8099917 showed no association in this cohort. PMID: 29130827
  67. evaluation of the association between TNF-alpha -308 G>A polymorphism and apical periodontitis phenotypes (Meta- Analysis) PMID: 29215675
  68. The present meta-analysis revealed that TNF alpha rs1800629, increased TNF alpha expression and secretion of nasal mucosal cells were associated with an increased risk of nasal polyposis. PMID: 29388847
  69. The heterozygous genotype GA of polymorphisms rs1800629 and rs361525 in the TNF promoter are associated with the risk of COPD. PMID: 29497291
  70. Results found MIF and TNFA mRNA expression increased in patients with high activity in relation to patients with moderate activity. These results suggest that both mRNA possibly have a role in the progress of the disease severity. PMID: 28965181
  71. the results indicate that TNFalpha downregulates REIC/Dkk3 expression in normal skin keratinocytes. PMID: 29512745
  72. TNFalpha-238 genetic polymorphism occurred more frequently in the individuals with Septic Arthroplasty failure - Periprosthetic Joint Infection. PMID: 29305046
  73. suggest that cardamonin inhibits TNF-alpha, IL-6 and NO production and downregulates iNOS expression and NF-kappaB activation. PMID: 29303022
  74. Genomic DNA samples extracted from the blood of 107 malaria-infected patients and 190 uninfected controls were analyzed, and a significant difference in the genotypic (p = 2.20E-16) and allelic frequencies (p = 2.20E-16) of the TNF-alpha (snp rs1800629) polymorphism was found. PMID: 28963676
  75. Data suggest that oxidative stress in PCOS (polycystic ovary syndrome) is associated with ovarian inflammation; biomarkers of oxidative stress [IL6, interleukin-6; IL8, interleukin-8; TNFA, tumor necrosis factor-alpha] are up-regulated in follicular fluid of women with PCOS as compared to control subjects. PMID: 28868943
  76. regulation of OSCAR by TNF-alpha and receptor activator of NF kappa beta ligand (RANKL) in pre-osteoclasts/osteoclasts PMID: 28555364
  77. TNF-alpha -238G>A polymorphism was involved in susceptibility to spinal tuerculosis, but not to pulmonary tuberculosis in Chinese polulation. PMID: 29430075
  78. The baseline concentrations of IL-6 and IL-1beta were significantly higher in the Major Depressive Disorder group than those in the control group. There was no significant difference of TNF-alpha between the two groups. PMID: 29587166
  79. The -221G>C polymorphism of MBL2, the -159C>T polymorphism of CD14 and the TNF-857 polymorphism of TNF-a are risk factors for spinal spinal tuberculosis (TB) and may be involved in the development of spinal TB in the Chinese population. These factors are indicators of susceptibility to spinal TB and require clinical attention. PMID: 29298876
  80. effect of IL-22 on Intestinal Epithelial Cells responses may not be in inducing CXCL8 by itself, but in enhancing TNF-alpha- and IL-1-induced CXCL8 secretion to augment the contribution of IECs to local inflammatory responses. PMID: 28656529
  81. Pro-inflammatory proteins S100A9 and tumor necrosis factor-alpha suppress erythropoietin elaboration in myelodysplastic syndromes PMID: 28983059
  82. Bio informatics analysis of 982 lung cancer patients revealed that higher expression of TNF- alpha was associated with low risk of cancer progression while overexpression of IGF-1 was correlated with high risk. Collectively, these results reveal that the cytokines in the tumor microenvironment differentially modulate radiation therapy through a variety of signaling mechanisms. PMID: 27344406
  83. TNFalpha may act reciprocally with DRA, leading to the development of intestinal inflammation. PMID: 29286110
  84. Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID: 29257244
  85. High TNFA expression may contribute for faster dopaminergic neuron degeneration PMID: 28844731
  86. Donor T cell-derived tumor necrosis factor-alpha (TNFalpha) was specifically found to directly impair the survival and division of transplanted hematopoietic stem and progenitor cells. PMID: 29263228
  87. rs1800629 polymorphism in TNF-alpha gene is associated with renal complications in type 2 diabetes mellitus subjects. PMID: 29042282
  88. The results suggest that the -308G > A TNF-alpha gene variant may play a role in the etiology of IUGR in the Polish population. PMID: 29664552
  89. The simultaneous delivery of multiple proinflammatory payloads to the cancer site conferred protective immunity against subsequent tumor challenges. A fully human homolog of IL2-F8-TNF(mut), which retained selectivity similar to its murine counterpart when tested on human material, may open new clinical applications for the immunotherapy of cancer. PMID: 28716814
  90. Findings suggest that the combinatorial complexity of TNFA and IL10 promoter polymorphisms impacts systemic lupus erythematosus susceptibility PMID: 29298134
  91. This meta-analysis supports previous findings that TNF-a -308G/A polymorphism may increase the risk of essential hypertension, at least in the Asian population. PMID: 29258412
  92. results suggest that TNF-alpha -308 G > A gene polymorphism significantly contributes to celiac disease susceptibility (meta-analysis). PMID: 27597177
  93. Serum interleukin-6 and tumor necrosis factor-alpha are associated with early graft regeneration after living donor liver transplantation PMID: 29649247
  94. TNF gene polymorphism -308G>A confers susceptibility to the development of nonalcoholic steatohepatitis in the Russian population; however the carriers of allele A are more sensitive to ursodeoxycholic acid therapy than carriers of GG genotype. PMID: 29181663
  95. TNF-alpha -857 C/C variant might represent protective effect against recurrent pregnancy loss (RPL) and the -857 C/T variant might be a genetic risk factor for the occurrence of RPL. Invariant differences in the prevalence of -511 C/T and -31 C/T polymorphisms and IL-1RN VNTR between RPL patients. PMID: 29949333
  96. Oxidized low density lipoprotein facilitates tumor necrosis factoralpha mediated chondrocyte death via its interaction with LOX1, and autophagy is involved in the mechanisms. PMID: 29039543
  97. Data suggest that TNF-alpha-308 polymorphism may influence the clinical features rather than susceptibility to ankylosing spondylitis in Han Chinese. PMID: 28610765
  98. TNF-alpha-3959T>C (rs1799964) was significantly associated with increased risk of intracranial aneurysm. PMID: 29027627
  99. TNF-alpha-G308A polymorphism may affect susceptibility to idiopathic nephrotic syndrome and might affect steroid response. PMID: 29190601
  100. Following arachidin-1 pretreatment, the H2O2-induced generation of reactive oxygen species was reduced. Therefore, the present results indicate that arachidin-1 suppresses TNF-alpha-induced inflammation in ECs through the upregulation of Nrf-2-related phase II enzyme expression. PMID: 29115410

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Involvement in disease Psoriatic arthritis (PSORAS)
Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11892

OMIM: 191160

KEGG: hsa:7124

STRING: 9606.ENSP00000398698

UniGene: Hs.241570

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