The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer. Avoid repeated freeze-thaw cycles.
Human AKT1 Primer Pair for ChIP
Species Reactivity :
Tested Applications :
60 ℃ anneal/extension for 45 sec
Quality Control :
AKT1 Primers were tested on DNA isolated from cross-linked Hela cells using formaldehyde and extracted with genomic DNA extraction kit. Real-time PCR was performed in triplicate on a serial dilution of input DNA (100ng, 20ng, 4ng, 0.8ng, 0.16ng and 0.032ng) using a real-time PCR detection system and SYBR®Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R2) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95 ℃ denaturation for 15 sec, 60 ℃ anneal/extension for 45 sec).