Recombinant ATPase asna-1 (asna-1)

Code CSB-YP339117CXY
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Source Yeast
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Code CSB-EP339117CXY
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Source E.coli
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Code CSB-EP339117CXY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339117CXY
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Source Baculovirus
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Code CSB-MP339117CXY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
asna-1
Uniprot No.
Alternative Names
asna-1; tag-205; ZK637.5ATPase asna-1; EC 3.6.-.-; Arsenical pump-driving ATPase; Arsenite-stimulated ATPase
Species
Caenorhabditis elegans
Expression Region
1-342
Target Protein Sequence
MSDQLEASIK NILEQKTLKW IFVGGKGGVG KTTCSCSLAA QLSKVRERVL LISTDPAHNI SDAFSQKFTK TPTLVEGFKN LFAMEIDSNP NGEGVEMGNI EEMLQNAAQN EGGSGGFSMG KDFLQSFAGG LPGIDEAMSF GEMIKLIDSL DFDVVVFDTA PTGHTLRLLQ FPTLLEKVFT KILSLQGMFG PMMNQFGGMF GMGGGSMNEM IEKMTTTLES VKKMNAQFKD PNCTTFVCVC IAEFLSLYET ERLIQELSKQ GIDTHNIIVN QLLFPDTDAN GTVSCRKCAS RQAIQSKYLT DIDELYEDFH VVKLPLLEAE VRGGPAILQF SERMVDPEAN KN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.
Gene References into Functions
  1. Data show that the parental gene, asna-1, was not targeted by miR-249, presumably because asna-1 is involved in insulin secretion; the cleavage of the asna-1 mRNA might be so detrimental and could be under selective pressure to escape miR-249 targeting. PMID: 23485825
  2. findings show that worms lacking activity of the asna-1 gene arrest growth reversibly at the L1 stage even when food is abundant; proposed that ASNA1 is an evolutionarily conserved modulator of insulin signaling PMID: 17289575
  3. As(III)- & Sb(III)-stimulated ArsA ATPase activities are not restricted to bacteria. asna-1 gene from C. elegans encodes functional ArsA ATPase whose activity is stimulated by As(III) & Sb(III), which is critical for As(III) & Sb(III) tolerance. PMID: 17419726
Subcellular Location
Cytoplasm. Endoplasmic reticulum.
Protein Families
ArsA ATPase family
Database Links

KEGG: cel:CELE_ZK637.5

STRING: 6239.ZK637.5

UniGene: Cel.9540

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