Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1)

Product Type Recombinant Protein
Uniprot No. P21947
Species Abutilon mosaic virus (isolate West India) (AbMV)
Gene Names AC1
Protein Names Recommended name: Replication-associated protein Short name= Rep EC= 2.7.7.- EC= 3.1.21.- Alternative name(s): Protein AC1 Protein AL1
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Subcellular Location Host nucleus
Protein Families Geminiviridae Rep protein family
Database Links

KEGG: vg:956371

Product Details for Different Expression Systems

Product Name Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1),Yeast
Code CSB-YP322055AAE
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPPPKKFRVQ AKNYFLTYPQ CSLTKDEALS QLQNLETPVN KKFIKICREL HENGEPHLHV LIQFEGKYQC TNNRFFDLVS PTRSAHFHPN IQGAKSSSDV KSYIDKDGDT AEWGEFQIDG RSARGGQQTA NDSYAKALNA GDVQSALNIL KEEQPKDYVL QNHNIRSNLE RIFAKAPEPW VPRFPLSSFT AVPEEMQEWA DDYFGSGSAA RPDRPLSLIV EGDSRTGKTM WARALGPHNY LSGHLDFNGR VYSNEVEYNV IDDVAPHYLK LKHWKELLGA QKDWQSNCKY GKPVQIKGGI PAIVLCNPGE GSSYKEYLDK EENTGLRNWT LKNAIFITLT APLYQEGTQA GQEEGH
Expression Region 1-356
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1),E.coli
Code CSB-EP322055AAE
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPPPKKFRVQ AKNYFLTYPQ CSLTKDEALS QLQNLETPVN KKFIKICREL HENGEPHLHV LIQFEGKYQC TNNRFFDLVS PTRSAHFHPN IQGAKSSSDV KSYIDKDGDT AEWGEFQIDG RSARGGQQTA NDSYAKALNA GDVQSALNIL KEEQPKDYVL QNHNIRSNLE RIFAKAPEPW VPRFPLSSFT AVPEEMQEWA DDYFGSGSAA RPDRPLSLIV EGDSRTGKTM WARALGPHNY LSGHLDFNGR VYSNEVEYNV IDDVAPHYLK LKHWKELLGA QKDWQSNCKY GKPVQIKGGI PAIVLCNPGE GSSYKEYLDK EENTGLRNWT LKNAIFITLT APLYQEGTQA GQEEGH
Expression Region 1-356
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1),Baculovirus
Code CSB-BP322055AAE
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPPPKKFRVQ AKNYFLTYPQ CSLTKDEALS QLQNLETPVN KKFIKICREL HENGEPHLHV LIQFEGKYQC TNNRFFDLVS PTRSAHFHPN IQGAKSSSDV KSYIDKDGDT AEWGEFQIDG RSARGGQQTA NDSYAKALNA GDVQSALNIL KEEQPKDYVL QNHNIRSNLE RIFAKAPEPW VPRFPLSSFT AVPEEMQEWA DDYFGSGSAA RPDRPLSLIV EGDSRTGKTM WARALGPHNY LSGHLDFNGR VYSNEVEYNV IDDVAPHYLK LKHWKELLGA QKDWQSNCKY GKPVQIKGGI PAIVLCNPGE GSSYKEYLDK EENTGLRNWT LKNAIFITLT APLYQEGTQA GQEEGH
Expression Region 1-356
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1),Mammalian cell
Code CSB-MP322055AAE
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPPPKKFRVQ AKNYFLTYPQ CSLTKDEALS QLQNLETPVN KKFIKICREL HENGEPHLHV LIQFEGKYQC TNNRFFDLVS PTRSAHFHPN IQGAKSSSDV KSYIDKDGDT AEWGEFQIDG RSARGGQQTA NDSYAKALNA GDVQSALNIL KEEQPKDYVL QNHNIRSNLE RIFAKAPEPW VPRFPLSSFT AVPEEMQEWA DDYFGSGSAA RPDRPLSLIV EGDSRTGKTM WARALGPHNY LSGHLDFNGR VYSNEVEYNV IDDVAPHYLK LKHWKELLGA QKDWQSNCKY GKPVQIKGGI PAIVLCNPGE GSSYKEYLDK EENTGLRNWT LKNAIFITLT APLYQEGTQA GQEEGH
Expression Region 1-356
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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