Recombinant Acholeplasma laidlawii SsrA-binding protein(smpB)

Code	CSB-YP433187ATX
Size	Pls inquire other sizes
Source	Yeast
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Code	CSB-EP433187ATX
Size	Pls inquire other sizes
Source	E.coli
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Code	CSB-EP433187ATX-B
Size	Pls inquire other sizes
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP433187ATX
Size	Pls inquire other sizes
Source	Baculovirus
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Code	CSB-MP433187ATX
Size	Pls inquire other sizes
Source	Mammalian cell
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Product Details
Target Data

Product Details

Purity	>85% (SDS-PAGE)
Target Names	smpB
Uniprot No.	A9NF97
Alternative Names	smpB; ACL_0406SsrA-binding protein; Small protein B
Species	Acholeplasma laidlawii (strain PG-8A)
Expression Region	1-149
Target Protein Sequence	MKIITQNKKA HFEYFIEQKF VAGIKLQGSE VKSIRAGKCS INEAYITFRN GEVFILNMHI AKFDASSIFN HEETRTRKLL LNRSEIDKLM GAQTRDGMTI IPLTVELHEG LIKVVIALAK GKKLFDKRET IKERDIKREQ QQSLKGKMR
Protein Length	full length protein
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet	Please contact us to get it.

Target Data

Function	Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.
Subcellular Location	Cytoplasm
Protein Families	SmpB family
Database Links	KEGG: acl:ACL_0406 STRING: 441768.ACL_0406