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| Product Type | Recombinant Protein |
| Uniprot No. | A9NF97 |
| Species | Acholeplasma laidlawii (strain PG-8A) |
| Gene Names | smpB |
| Protein Names | Recommended name: SsrA-binding protein |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Function | Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation. |
| Subcellular Location | Cytoplasm |
| Protein Families | SmpB family |
| Database Links |
KEGG: acl:ACL_0406 STRING: 441768.ACL_0406 |
| Product Name | Recombinant Acholeplasma laidlawii SsrA-binding protein(smpB),Yeast |
| Code | CSB-YP433187ATX |
| Size | Pls inquiry other sizes |
| Source | Yeast |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
|
| Sequence | MKIITQNKKA HFEYFIEQKF VAGIKLQGSE VKSIRAGKCS INEAYITFRN GEVFILNMHI AKFDASSIFN HEETRTRKLL LNRSEIDKLM GAQTRDGMTI IPLTVELHEG LIKVVIALAK GKKLFDKRET IKERDIKREQ QQSLKGKMR |
| Expression Region | 1-149 |
| Protein Description | full length protein |
| Storage Buffer | Tris-based buffer,50% glycerol |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Product Name | Recombinant Acholeplasma laidlawii SsrA-binding protein(smpB),E.coli |
| Code | CSB-EP433187ATX |
| Size | Pls inquiry other sizes |
| Source | E.coli |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged and C-terminal Myc-tagged N-terminal His-SUMO-tagged and C-terminal Myc-tagged N-terminal His-B2M-tagged and C-terminal Myc-tagged N-terminal His-GST-tagged and C-terminal Myc-tagged N-terminal His-tagged N-terminal His-SUMO-tagged N-terminal His-B2M-tagged N-terminal His-GST-tagged N-terminal GST-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
|
| Sequence | MKIITQNKKA HFEYFIEQKF VAGIKLQGSE VKSIRAGKCS INEAYITFRN GEVFILNMHI AKFDASSIFN HEETRTRKLL LNRSEIDKLM GAQTRDGMTI IPLTVELHEG LIKVVIALAK GKKLFDKRET IKERDIKREQ QQSLKGKMR |
| Expression Region | 1-149 |
| Protein Description | full length protein |
| Storage Buffer | Tris-based buffer,50% glycerol |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Product Name | Recombinant Acholeplasma laidlawii SsrA-binding protein(smpB),Baculovirus |
| Code | CSB-BP433187ATX |
| Size | Pls inquiry other sizes |
| Source | Baculovirus |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged and C-terminal Myc-tagged N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
|
| Sequence | MKIITQNKKA HFEYFIEQKF VAGIKLQGSE VKSIRAGKCS INEAYITFRN GEVFILNMHI AKFDASSIFN HEETRTRKLL LNRSEIDKLM GAQTRDGMTI IPLTVELHEG LIKVVIALAK GKKLFDKRET IKERDIKREQ QQSLKGKMR |
| Expression Region | 1-149 |
| Protein Description | full length protein |
| Storage Buffer | Tris-based buffer,50% glycerol |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Product Name | Recombinant Acholeplasma laidlawii SsrA-binding protein(smpB),Mammalian cell |
| Code | CSB-MP433187ATX |
| Size | Pls inquiry other sizes |
| Source | Mammalian cell |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged and C-terminal Myc-tagged N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
|
| Sequence | MKIITQNKKA HFEYFIEQKF VAGIKLQGSE VKSIRAGKCS INEAYITFRN GEVFILNMHI AKFDASSIFN HEETRTRKLL LNRSEIDKLM GAQTRDGMTI IPLTVELHEG LIKVVIALAK GKKLFDKRET IKERDIKREQ QQSLKGKMR |
| Expression Region | 1-149 |
| Protein Description | full length protein |
| Storage Buffer | Tris-based buffer,50% glycerol |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
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