Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 23 (CIPK23)

Code CSB-YP842405DOA
MSDS
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Source Yeast
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Code CSB-EP842405DOA
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Source E.coli
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Code CSB-EP842405DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842405DOA
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Source Baculovirus
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Code CSB-MP842405DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CIPK23
Uniprot No.
Alternative Names
CIPK23; LKS1; PKS17; SnRK3.23; At1g30270; F12P21.6CBL-interacting serine/threonine-protein kinase 23; EC 2.7.11.1; Protein LOW-K(+)-SENSITIVE 1; SNF1-related kinase 3.23; SOS2-like protein kinase PKS17
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-482
Target Protein Sequence
MASRTTPSRS TPSRSTPSGS SSGGRTRVGK YELGRTLGEG TFAKVKFARN VENGDNVAIK VIDKEKVLKN KMIAQIKREI STMKLIKHPN VIRMFEVMAS KTKIYFVLEF VTGGELFDKI SSNGRLKEDE ARKYFQQLIN AVDYCHSRGV YHRDLKPENL LLDANGALKV SDFGLSALPQ QVREDGLLHT TCGTPNYVAP EVINNKGYDG AKADLWSCGV ILFVLMAGYL PFEDSNLTSL YKKIFKAEFT CPPWFSASAK KLIKRILDPN PATRITFAEV IENEWFKKGY KAPKFENADV SLDDVDAIFD DSGESKNLVV ERREEGLKTP VTMNAFELIS TSQGLNLGSL FEKQMGLVKR KTRFTSKSSA NEIVTKIEAA AAPMGFDVKT NNYKMKLTGE KSGRKGQLAV ATEVFQVAPS LYMVEMRKSG GDTLEFHKFY KNLTTGLKDI VWKTIDEEKE EGTDGGGTNG AMANRTIAKQ ST
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein leads to activation of the kinase in a calcium-dependent manner. Downstream of CBL1, CBL2, CBL3 and CBL9, regulates by phosphorylation the K(+) conductance and uptake of AKT1 in low K(+) condition, in response to calcium signaling and during the stomatal opening regulation by monitoring the turgor pressure in guard cells. In response to low nitrate concentration, phosphorylates NRT1.1, switching it from a low-affinity nitrate transporter to a high-affinity transporter. Confers tolerance to low potassium conditions. Involved in drought sensitivity and leaf transpiration.
Gene References into Functions
  1. CIPK23 occupies a key position in controlling ion balance and ion homeostasis in the plant cell. PMID: 28188265
  2. Studies highlight that calcium-dependent CBL-CIPK pathway is involved in response to iron deficiency in Arabidopsis. [CIPK23] PMID: 26993237
  3. CIPK23 rendered Arabidopsis mutants hypersensitive to iron deficiency. PMID: 26993237
  4. protein kinase CIPK23, encoded by the LKS1 gene, regulates K+ uptake under low-K+ conditions PMID: 16814720
Subcellular Location
Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when associated with AKT1, CBL1 and CBL9.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
In seedlings, mostly in vascular bundles, and in roots, especially in cortex and endodermis cells. In adult plants, mostly expressed in flowers, and, to a lower extent, in roots, leaves, stems and siliques, particularly in vascular tissues. Also detected
Database Links

KEGG: ath:AT1G30270

STRING: 3702.AT1G30270.1

UniGene: At.17201

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