Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 9 (CIPK9)

Code CSB-YP888947DOA
MSDS
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Source Yeast
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Code CSB-EP888947DOA
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Source E.coli
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Code CSB-EP888947DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888947DOA
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Source Baculovirus
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Code CSB-MP888947DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CIPK9
Uniprot No.
Alternative Names
CIPK9; PKS6; SnRK3.12; At1g01140; F6F3.28; T25K16.13CBL-interacting serine/threonine-protein kinase 9; EC 2.7.11.1; SNF1-related kinase 3.12; SOS2-like protein kinase PKS6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-447
Target Protein Sequence
MSGSRRKATP ASRTRVGNYE MGRTLGEGSF AKVKYAKNTV TGDQAAIKIL DREKVFRHKM VEQLKREIST MKLIKHPNVV EIIEVMASKT KIYIVLELVN GGELFDKIAQ QGRLKEDEAR RYFQQLINAV DYCHSRGVYH RDLKPENLIL DANGVLKVSD FGLSAFSRQV REDGLLHTAC GTPNYVAPEV LSDKGYDGAA ADVWSCGVIL FVLMAGYLPF DEPNLMTLYK RICKAEFSCP PWFSQGAKRV IKRILEPNPI TRISIAELLE DEWFKKGYKP PSFDQDDEDI TIDDVDAAFS NSKECLVTEK KEKPVSMNAF ELISSSSEFS LENLFEKQAQ LVKKETRFTS QRSASEIMSK MEETAKPLGF NVRKDNYKIK MKGDKSGRKG QLSVATEVFE VAPSLHVVEL RKTGGDTLEF HKFYKNFSSG LKDVVWNTDA AAEEQKQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Involved in K(+) homeostasis under low-K(+) stress.
Gene References into Functions
  1. CIPK9 gene was responsive to abiotic stress conditions, and its transcript was inducible in both roots and shoots by potassium deprivation. Disruption of CIPK9 function rendered the mutant plants hypersensitive to low potassium media. PMID: 17486125
Subcellular Location
Cytoplasm. Nucleus. Note=Targeted to the tonoplast when interacting with CBL2 or CBL3.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Expressed at low levels in roots and shoots. Detected in root vascular bundles and in the leaf vascular tissue and hydathode, but not in root tips.
Database Links

KEGG: ath:AT1G01140

STRING: 3702.AT1G01140.3

UniGene: At.11899

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