Recombinant Arabidopsis thaliana COP9 signalosome complex subunit 3 (CSN3)

Code CSB-YP855420DOA
MSDS
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Source Yeast
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Code CSB-EP855420DOA
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Source E.coli
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Code CSB-EP855420DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855420DOA
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Source Baculovirus
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Code CSB-MP855420DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSN3
Uniprot No.
Alternative Names
CSN3; FUS11; At5g14250; F18O22.40; COP9 signalosome complex subunit 3; Signalosome subunit 3; Protein FUSCA 11
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-429
Target Protein Sequence
MIGAVNSVEA VITSIQGLSG SPEDLSALHD LLRGAQDSLR AEPGVNFSTL DQLDASKHSL GYLYFLEVLT CGPVSKEKAA YEIPIIARFI NSCDAGQIRL ASYKFVSLCK ILKDHVIALG DPLRGVGPLL NAVQKLQVSS KRLTALHPDV LQLCLQAKSY KSGFSILSDD IVEIDQPRDF FLYSYYGGMI CIGLKRFQKA LELLYNVVTA PMHQVNAIAL EAYKKYILVS LIHNGQFTNT LPKCASTAAQ RSFKNYTGPY IELGNCYNDG KIGELEALVV ARNAEFEEDK NLGLVKQAVS SLYKRNILRL TQKYLTLSLQ DIANMVQLGN AKEAEMHVLQ MIQDGQIHAL INQKDGMVRF LEDPEQYKSS EMIEIMDSVI QRTIGLSKNL LAMDESLSCD PLYLGKVGRE RQRYDFGDDF DTVPQKFSM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.
Gene References into Functions
  1. CSN3 functions in a distinct protein complex that is required for proper auxin signaling. PMID: 23762492
  2. Data suggest that Arabidopsis csn mutants undergo DNA damage, which might be the cause of the delay in G2 cell cycle progression. PMID: 18434413
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
CSN3 family
Database Links

KEGG: ath:AT5G14250

STRING: 3702.AT5G14250.1

UniGene: At.5163

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