Recombinant Arabidopsis thaliana Defensin-like protein 16 (PDF1.2A)

Code CSB-YP887761DOA
MSDS
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Source Yeast
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Code CSB-EP887761DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887761DOA
MSDS
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Source Baculovirus
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Code CSB-MP887761DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDF1.2A
Uniprot No.
Alternative Names
PDF1.2A; LCR77; PDF1.2; At5g44420; MFC16.8Defensin-like protein 16; Low-molecular-weight cysteine-rich protein 77; Protein LCR77; Plant defensin 1.2; Plant defensin 1.2a
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
30-80
Target Protein Sequence
Q KLCEKPSGTW SGVCGNSNAC KNQCINLEGA KHGSCNYVFP AHKCICYVPC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Confers broad-spectrum resistance to pathogens. Has antifungal activity in vitro.
Gene References into Functions
  1. Natural variation in jasmonic acid-induced PDF1.2 expression is associated with basal resistance against the nectrophic fungus Plectosphaerella cucumerina. PMID: 21414016
  2. ORA59 and two functionally equivalent GCC box binding sites form the module that enables the PDF1.2 gene to respond synergistically to simultaneous activation of the jasmonate and ethylene signaling pathways PMID: 21246258
  3. Growth and infection of Phakopsora pachyrhizi hyphae in Arabidopsis were terminated at the mesophyll boundary, and associated with expression of PDF1.2. PMID: 18842092
Subcellular Location
Secreted.
Protein Families
DEFL family
Tissue Specificity
Predominantly expressed in leaves.
Database Links

KEGG: ath:AT5G44420

STRING: 3702.AT5G44420.1

UniGene: At.27168

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