Recombinant Arabidopsis thaliana Epithiospecifier protein (ESP)

Code CSB-YP844588DOA
MSDS
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Source Yeast
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Code CSB-EP844588DOA
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Source E.coli
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Code CSB-EP844588DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844588DOA
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Source Baculovirus
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Code CSB-MP844588DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ESP
Uniprot No.
Alternative Names
ESP; ESR; TASTY; At1g54040; F15I1.12; Epithiospecifier protein; AtESP; Protein EPITHIOSPECIFYING SENESCENCE REGULATOR; AtESR
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-341
Target Protein Sequence
MAPTLQGQWI KVGQKGGTGP GPRSSHGIAA VGDKLYSFGG ELTPNKHIDK DLYVFDFNTQ TWSIAQPKGD APTVSCLGVR MVAVGTKIYI FGGRDENRNF ENFRSYDTVT SEWTFLTKLD EVGGPEARTF HSMASDENHV YVFGGVSKGG TMNTPTRFRT IEAYNIADGK WAQLPDPGDN FEKRGGAGFA VVQGKIWVVY GFATSIVPGG KDDYESNAVQ FYDPASKKWT EVETTGAKPS ARSVFAHAVV GKYIIIFAGE VWPDLNGHYG PGTLSNEGYA LDTETLVWEK LGEEGAPAIP RGWTAYTAAT VDGKNGLLMH GGKLPTNERT DDLYFYAVNS A
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Converts glucosinolates both to epithionitriles and to simple nitriles in the presence of myrosinase. Promotes the formation of epithionitriles after hydrolysis of alkenylglucosinolates containing a terminal double bond. Mediates indol-3-acetonitrile (IACN) production from indol-3-ylmethyl glucosinolate. Acts as a negative regulator of senescence.
Gene References into Functions
  1. expression during growth cycle in different organs and alternative splicing of epithiospecifier protein PMID: 22228407
  2. ESP-expressing cells in various organs and several developmental stages of different Arabidopsis thaliana ecotypes are identified by immunolocalization. PMID: 17390109
Subcellular Location
Cytoplasm. Nucleus. Note=Brought to the nucleus after interaction with WRKY53.
Tissue Specificity
Expressed in epidermal cells of all above-ground organs except the anthers, in cambial cells of leaf and stem vascular bundles, and in glucosinolates rich S-cells found in stems just below the inflorescence. Absent from roots.
Database Links

KEGG: ath:AT1G54040

STRING: 3702.AT1G54040.2

UniGene: At.24494

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