Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ESR1 (ESR1)

Code CSB-YP882808DOA
MSDS
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Source Yeast
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Code CSB-EP882808DOA
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Source E.coli
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Code CSB-EP882808DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882808DOA
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Source Baculovirus
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Code CSB-MP882808DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ESR1
Uniprot No.
Alternative Names
ESR1; DRN; ERF089; At1g12980; F3F19.1Ethylene-responsive transcription factor ESR1; Protein DORNROSCHEN; Protein ENHANCER OF SHOOT REGENERATION 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-328
Target Protein Sequence
MEKALRNFTE STHSPDPNPL TKFFTEPTAS PVSRNRKLSS KDTTVTIAGA GSSTTRYRGV RRRPWGRYAA EIRDPMSKER RWLGTFDTAE QAACAYDSAA RAFRGAKART NFTYPTAVIM PEPRFSFSNK KSSPSARCPL PSLPLDSSTQ NFYGAPAAQR IYNTQSIFLR DASCSSRKTT PYNNSFNGSS SSYSASKTAC VSYSENENNE SFFPEESSDT GLLQEVVQEF LKKNRGVPPS PPTPPPVTSH HDNSGYFSNL TIYSENMVQE TKETLSSKLD RYGNFQANDD GVRAVADGGL SLGSNEWGYQ EMLMYGTQLG CTCRRSWG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates gene expression patterns in meristems and thus modulates organ development. Required for correct embryo patterning and cotyledon organogenesis. Modulates auxin signaling pathway in early embryos. Involved in the cytokinin signaling pathway that promotes shoot regeneration. Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways.
Gene References into Functions
  1. WIND1 directly binds the ESR1 promoter and activates tts expression. PMID: 28011694
  2. Study shows that DRN and DRNL promote cell proliferation, most probably via the G1-S transition. PMID: 27277595
  3. These results define new roles for KH-domain containing proteins with ESR1 unlinking jasmonic acid-mediated growth and defense responses. PMID: 25985302
  4. Although DRN and DRNL transgenic lines are both activated in surface cells, their promoter activity marks different cell identities. PMID: 23011125
  5. ESR1 is expressed both prior to and during shoot formation, whereas ESR2 is expressed only during shoot formation. PMID: 21600396
  6. DRN and DRNL act cell-autonomously and are co-expressed in the early globular embryo, whereas expression patterns diverge during later stages of embryogeny PMID: 21161330
  7. Drn Drnl double mutants with weak or strong drnl alleles show more highly penetrant and extreme phenotypes, including a pin-like embryo without cotyledons, confirming a high degree of functional redundancy for the two genes in embryo patterning. PMID: 17376809
  8. while overexpression of ESR1 fused with a strong repression domain, SRDX, inhibited shoot regeneration. These results suggest that ESR1 induces shoot regeneration through its transactivating ability PMID: 18771931
  9. interaction of BIM1 with DRN, involved with auxin signalling, represents a possible point of hormonal crosstalk in embryonic patterning and the first example of an interaction of components of the auxin and BR signalling pathways PMID: 18830673
  10. MP binds in vivo to two AuxRE-spanning fragments in the DRN promoter; MP is required for expression of DRN in cotyledon tips. Hence, DRN represents a direct target of MP and functions downstream of MP in cotyledon development PMID: 19369397

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed in shoot apical and floral meristems, and in organ primordia. Transient expression during shoot regeneration.
Database Links

KEGG: ath:AT1G12980

STRING: 3702.AT1G12980.1

UniGene: At.28308

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