Recombinant Arabidopsis thaliana Guanine nucleotide-binding protein subunit beta-like protein C (RACK1C)

Code CSB-YP878602DOA
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Source Yeast
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Code CSB-EP878602DOA
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Source E.coli
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Code CSB-EP878602DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878602DOA
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Source Baculovirus
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Code CSB-MP878602DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RACK1C
Uniprot No.
Alternative Names
RACK1C; At3g18130; MRC8.11Receptor for activated C kinase 1C; Guanine nucleotide-binding protein subunit beta-like protein C
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-326
Target Protein Sequence
MAEGLVLKGI MRAHTDIVTA IATPIDNSDI IVTASRDKSI ILWKLTKDDK SYGVAQRRLT GHSHFVEDVV LSSDGQFALS GSWDGELRLW DLATGETTRR FVGHTKDVLS VAFSTDNRQI VSASRDRTIK LWNTLGECKY TISEGDGHKE WVSCVRFSPN TLVPTIVSAS WDKTVKVWNL QNCKLRNSLV GHSGYLNTVA VSPDGSLCAS GGKDGVILLW DLAEGKKLYS LEAGSIIHSL CFSPNRYWLC AATENSIRIW DLESKSVVED LKVDLKSEAE KNEGGVGTGN QKKVIYCTSL NWSADGSTLF SGYTDGVVRV WGIGRY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Minor component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways. Involved in multiple hormone responses and developmental processes. MAPK cascade scaffolding protein involved in the protease IV and ArgC signaling pathway but not the flg22 pathway.
Gene References into Functions
  1. These results indicate the intertwined structure of a relatively small and stable protein core and the remaining regions of different levels of flexibility. PMID: 24591271
  2. Receptor for activated C kinase 1C, a WD40 repeat protein, was up-regulated in the cbl1 null mutant, and two rack1c mutant lines showed decreased tolerance to salt stress, suggesting that RACK1C plays a role in salt stress resistance. PMID: 22002954
  3. RACK1 may be required for normal production of 60S and 80S ribosomes and that its action in these processes may be regulated by abscissic acid. [RACK1C] PMID: 21098678
  4. The first crystal structure of a RACK1 protein, RACK1 isoform A from Arabidopsis thaliana, at 2.4 A resolution is presented. PMID: 18715992
  5. RACK1C (At3g18130) is functionally equivalent to RACK1A, and is positively regulated by other RACK1 genes. PMID: 18947417
  6. RACK1 genes act redundantly to negatively regulate abscisic acid responses during seed germination and early seedling development PMID: 19584117
  7. An enhanced effect on ABA hypersensitivity was observed between rack1a and gpa1 or agb1 mutants in the ABA inhibition of cotyledon greening and root growth. RACK1 and AGB1 may not physically interact with each other. PMID: 19651700

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Protein Families
WD repeat G protein beta family, Ribosomal protein RACK1 subfamily
Tissue Specificity
Widely expressed.
Database Links

KEGG: ath:AT3G18130

STRING: 3702.AT3G18130.1

UniGene: At.22741

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