Recombinant Arabidopsis thaliana Homeobox protein SHOOT MERISTEMLESS (STM)

Code CSB-YP657125DOA
MSDS
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Source Yeast
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Code CSB-EP657125DOA
MSDS
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Source E.coli
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Code CSB-EP657125DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657125DOA
MSDS
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Source Baculovirus
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Code CSB-MP657125DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STM
Uniprot No.
Alternative Names
STM; At1g62360; F2401.9; Homeobox protein SHOOT MERISTEMLESS
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-382
Target Protein Sequence
MESGSNSTSC PMAFAGDNSD GPMCPMMMMM PPIMTSHQHH GHDHQHQQQE HDGYAYQSHH QQSSSLFLQS LAPPQGTKNK VASSSSPSSC APAYSLMEIH HNEIVAGGIN PCSSSSSSAS VKAKIMAHPH YHRLLAAYVN CQKVGAPPEV VARLEEACSS AAAAAASMGP TGCLGEDPGL DQFMEAYCEM LVKYEQELSK PFKEAMVFLQ RVECQFKSLS LSSPSSFSGY GETAIDRNNN GSSEEEVDMN NEFVDPQAED RELKGQLLRK YSGYLGSLKQ EFMKKRKKGK LPKEARQQLL DWWSRHYKWP YPSEQQKLAL AESTGLDQKQ INNWFINQRK RHWKPSEDMQ FVVMDATHPH HYFMDNVLGN PFPMDHISST ML
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for shoot apical meristem (SAM) formation during embryogenesis. Negatively regulates ASYMMETRIC LEAVES1 (AS1) and ASYMMETRIC LEAVES2 (AS2 or LBD6). Probably binds to the DNA sequence 5'-TGAC-3'. Binds to RNA.
Gene References into Functions
  1. STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. PMID: 29650590
  2. These observations suggest that the MYB96-STM module contributes to enhancing plant tolerance to drought stress. PMID: 27448723
  3. The authors test and demonstrate that STM expression is induced after micromechanical perturbations. PMID: 26623515
  4. The ELK domain has a role in the posttranslational regulation of STM by affecting the nuclear localization of STM. PMID: 25524441
  5. STM has dual cellular and meristem-organisational functions that are differentially represented in the class-1 KNOX gene family and have differing requirements for cytokinin (CK) and CYCD3. PMID: 23573875
  6. mediates the regulatory network of cell wall and lignin synthesis PMID: 23242073
  7. STM is targeted into the nuclear compartment through heterodimerization with Bel1-like homeodomain partner proteins. PMID: 16513846
  8. Heterodimers with PENNYWISE or POUND-FOOLISH control floral specification, internode patterning and the maintenance of boundaries between initiating floral primordia and the inflorescence meristem. PMID: 16741748
  9. STM provides the critical KNOX function in the development of both vegetative and reproductive meristematic tissues. PMID: 17461793
  10. regulatory point of leaf development mediated through a conserved, noncoding sequence in STM genes. PMID: 17898165
  11. the DNA-binding properties of the complex formed by the Arabidopsis TALE homeodomain (HD) proteins STM and BLH3 PMID: 19442701

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Subcellular Location
Nucleus. Cell junction, plasmodesma. Cytoplasm. Endosome. Cell membrane.
Protein Families
TALE/KNOX homeobox family
Tissue Specificity
Expressed in all four types of shoot apical meristems (SAM) i.e. in vegetative, axillary, inflorescence and floral.
Database Links

KEGG: ath:AT1G62360

STRING: 3702.AT1G62360.1

UniGene: At.10801

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