Recombinant Arabidopsis thaliana Meiotic recombination protein DMC1 homolog (LIM15)

Code CSB-YP657183DOA
MSDS
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Source Yeast
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Code CSB-EP657183DOA
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Source E.coli
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Code CSB-EP657183DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657183DOA
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Source Baculovirus
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Code CSB-MP657183DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DMC1
Uniprot No.
Alternative Names
DMC1; LIM15; At3g22880; F5N5.6Meiotic recombination protein DMC1 homolog; AtDMC1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-344
Target Protein Sequence
MMASLKAEET SQMQLVEREE NDEDEDLFEM IDKLIAQGIN AGDVKKLQEA GIHTCNGLMM HTKKNLTGIK GLSEAKVDKI CEAAEKIVNF GYMTGSDALI KRKSVVKITT GCQALDDLLG GGIETSAITE AFGEFRSGKT QLAHTLCVTT QLPTNMKGGN GKVAYIDTEG TFRPDRIVPI AERFGMDPGA VLDNIIYARA YTYEHQYNLL LGLAAKMSEE PFRILIVDSI IALFRVDFTG RGELADRQQK LAQMLSRLIK IAEEFNVAVY MTNQVIADPG GGMFISDPKK PAGGHVLAHA ATIRLLFRKG KGDTRVCKVY DAPNLAEAEA SFQITQGGIA DAKD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks. Mediates interhomolog recombination during meiosis.
Gene References into Functions
  1. KLU is required for normal DMC1 expression pattern in ovules undergoing megasporogenesis. PMID: 25182975
  2. The DMC1 siRNA interfered with meiotic progression and, consequently, the flowers were at least partially sterile. PMID: 25039964
  3. Our data demonstrate that RAD51 plays a supporting role for DMC1 in meiotic recombination in the flowering plant, Arabidopsis. PMID: 24086145
  4. Results demonstrate that DMC1 functions independently and spatially separated from RAD51 during meiosis and that ATR is an integral part of the regular meiotic program. PMID: 22589466
  5. Establishment and stabilisation of pairing of homologous centromeric and pericentromeric regions depends principally upon DMC1, while pairing and synapsis of euchromatic chromosome arms of homologues requires the presence of RAD51 . PMID: 22532804
  6. AtRAD51 may be required to ensure the fidelity of homologous recombination in the interchromosomal exchanges initiated by AtDMC1. PMID: 22066484
  7. AtBRCA2 is required for proper meiotic synapsis and mediates the recruitment of AtRAD51 and AtDMC1. PMID: 22077663

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Subcellular Location
Nucleus.
Protein Families
RecA family, DMC1 subfamily
Tissue Specificity
Expressed in mitotic and/or meiotic tissues. Expressed in roots, leaves and anthers and carpels of young fower buds.
Database Links

KEGG: ath:AT3G22880

STRING: 3702.AT3G22880.1

UniGene: At.50216

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