Recombinant Arabidopsis thaliana Methyl-CpG-binding domain-containing protein 7 (MBD7)

Code CSB-YP887775DOA
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Source Yeast
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Code CSB-EP887775DOA
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Source E.coli
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Code CSB-EP887775DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887775DOA
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Source Baculovirus
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Code CSB-MP887775DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MBD7
Uniprot No.
Alternative Names
MBD7; At5g59800; MMN10.2; Methyl-CpG-binding domain-containing protein 7; AtMBD7; MBD07; Methyl-CpG-binding protein MBD7
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-306
Target Protein Sequence
MQTRSSSSPS ANHRRETQLQ IADPTSFCGK IMPGWTVVNR PRSSNNGVVD TYFIEPGTGR QFSSLEAIHR HLAGEVNDRR LTRAGSFFQD KTRVYEGSRT KQDHCGVEYA SKGFRLPRGW SVEEVPRKNS HYIDKYYVER KTGKRFRSLV SVERYLRESR NSIEQQLRVL QNRRGHSKDF RLPDGWIVEE KPRRSSSHID RSYIEPGTGN KFRSMAAVER YLISVGNITL DSVSMVHSER LPLLMNRNGI RFQSEVIDPN PPKKVKWVLT GSGGNMFTAN VRGSNVSSLV KHTWSEAFVS LIEDRS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. May directly affect chromatin structure by inducing intra- and inter- chromatin compaction via bridging over multiple methylated CpG sites. Acts as an anti-silencing factor that prevents DNA hypermethylation and gene repression. Requires high mCG density for binding. Recognizes preferentially mCGs located in transposable elements. Required for active DNA demethylation. Prefers to target genomic loci around chromocenters.
Gene References into Functions
  1. MBD7 binds to genomic regions with a high density of CG methylation.MBD7 physically associates with IDM3, and with IDM1 and IDM2 proteins.MBD7 and IDM3 prevent DNA hypermethylation and gene silencing. PMID: 25684209
  2. MBD7 is required for active DNA demethylation and antisilencing of the genomic loci with high densities of DNA methylation in Arabidopsis. PMID: 25593350
  3. The C-terminal, StkC motif plays an important role in fastening AtMBD7 to its chromosomal, CpG-methylated sites. PMID: 19647732
  4. The characterization of proteins interacting with MBD7, especially PRMT11, is reported. PMID: 17711414
  5. poly-MBD proteins can directly affect chromatin structure by inducing intra- and inter-chromatin compaction via bridging over multiple methylated CpG sites PMID: 18211904

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Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Expressed in leaves, buds, flowers, stems, siliques, mature seeds and roots.
Database Links

KEGG: ath:AT5G59800

STRING: 3702.AT5G59800.1

UniGene: At.29205

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