Recombinant Arabidopsis thaliana Mitogen-activated protein kinase kinase 6 (MKK6)

Code CSB-YP013415DOA
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Source Yeast
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Code CSB-EP013415DOA
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Source E.coli
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Code CSB-EP013415DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013415DOA
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Source Baculovirus
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Code CSB-MP013415DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MKK6
Uniprot No.
Alternative Names
MKK6; ANQ1; At5g56580; MIK19.2Mitogen-activated protein kinase kinase 6; AtMKK6; MAP kinase kinase 6; EC 2.7.12.2; Protein Arabidopsis NQK1 homolog
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-356
Target Protein Sequence
MVKIKSNLKQ LKLSVPAQES PISSFLTASG TFHDGDFLLN QKGLRLTSDE KQSRQSDSKE LDFEITAEDL ETVKVIGKGS GGVVQLVRHK WVGKFFAMKV IQMNIQEEIR KQIVQELKIN QASSQCPHVV VCYHSFYHNG AFSLVLEYMD RGSLADVIRQ VKTILEPYLA VVCKQVLLGL VYLHNERHVI HRDIKPSNLL VNHKGEVKIS DFGVSASLAS SMGQRDTFVG TYNYMSPERI SGSTYDYSSD IWSLGMSVLE CAIGRFPYLE SEDQQNPPSF YELLAAIVEN PPPTAPSDQF SPEFCSFVSA CIQKDPPARA SSLDLLSHPF IKKFEDKDID LGILVGTLEP PVNYLR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. MKK6-MPK13 module positively regulates lateral root formation. Phosphorylates and activates MPK4. Activates MPK5 and MPK13 in vitro.
Gene References into Functions
  1. AtMKK6-AtMPK13 module positively regulates lateral root formation. PMID: 21904115
  2. Plants with mutations in the MKK6/ANQ gene exhibit severe defects in cytokinesis. PMID: 20802223
Subcellular Location
Cytoplasm, cytoskeleton, phragmoplast. Note=Localized at the equatorial plane of the phragmoplast.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
Expressed in roots, stems and flower buds. Higher levels in shoot apices and flowers.
Database Links

KEGG: ath:AT5G56580

STRING: 3702.AT5G56580.1

UniGene: At.29375

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