Recombinant Arabidopsis thaliana Transcription repressor KAN1 (KAN1)

Code CSB-YP842410DOA
MSDS
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Source Yeast
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Code CSB-EP842410DOA
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Source E.coli
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Code CSB-EP842410DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842410DOA
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Source Baculovirus
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Code CSB-MP842410DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KAN1
Uniprot No.
Alternative Names
KAN1; KAN; At5g16560; MQK4.31; Transcription repressor KAN1; Protein KANADI 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-403
Target Protein Sequence
MSMEGVFLEK TKTNTTTTLP DLSLHISLPD IHQYHHNESS KESSRRSSQL ENNNRSSNFE LSLSHHNHPT ARIFHCPDRR TLNLPHQQHY NNPIINGVHQ RVDESEISNL HRPIRGIPVY HNRSFPFHQQ NSSLPSLGGG DMDQISILNS SSGYNNAYRS LQSSPRLKGV PLHHHHHHNQ YGVVGSSDSS SPHHHNHHHH GMIRSRFLPK MPTKRSMRAP RMRWTSSLHA RFVHAVELLG GHERATPKSV LELMDVKDLT LAHVKSHLQM YRTVKTTNKP AASSDGSGEE EMGINGNEVH HQSSTDQRAQ SDDTSLHQET DISSTQPRWS NSSRETWPLS NNCSSDIDTM IRTSSTSMIS HYQRSSIQNQ EQRSNDQAKR CGNLSCENPS LEFTLGRPDW HEK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional repressor that regulates lateral organ polarity. Promotes lateral organ abaxial identity by repressing the adaxial regulator ASYMMETRIC LEAVES2 (AS2) in abaxial cells. Required for abaxial identity in both leaves and carpels. Functions with KAN2 in the specification of polarity of the ovule outer integument. Regulates cambium activity by repressing the auxin efflux carrier PIN1. Plays a role in lateral root formation and development.
Gene References into Functions
  1. KAN1 acts upstream of several genes encoding auxin biosynthetic enzymes.KAN1 plays a role in shade-avoidance responses.KAN1 activity suppresses auxin synthesis and signaling. PMID: 26246448
  2. HD-ZIPIII and KANADI regulate tissue-specific transcription factors involved in subsidiary developmental decisions, nearly all major hormone pathways, and new actors (such as indeterminate domain4) in the ad/abaxial regulatory network. PMID: 24076978
  3. HD-ZIPIII and KANADI transcription factors pattern auxin accumulation and responsiveness in the embryo. PMID: 24464295
  4. a set of potential direct target genes of KAN1, were identified. PMID: 24155946
  5. Leaf polarity is established by the opposed actions of HD-ZIPIII and KANADI transcription factors. PMID: 22578006
  6. Class III HD-ZIP and KANADI transcription factors control cambium activity: KANADI proteins by acting on auxin transport, and Class III HD-ZIP proteins by promoting axial cell elongation and xylem differentiation. PMID: 20179097
  7. As KANADI is not required for either ETT or ARF4 transcription, and their overexpression cannot rescue kanadi mutants, cooperative activity is implied. PMID: 16199616
  8. KAN1 acts as a transcriptional repressor and that mutually repressive interactions between KAN1 and AS2 contribute to the establishment of adaxial-abaxial polarity in plants PMID: 18849474

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in developing phloem and lateral root.
Database Links

KEGG: ath:AT5G16560

STRING: 3702.AT5G16560.1

UniGene: At.6466

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