Recombinant Arabidopsis thaliana Transcriptional regulator SUPERMAN (SUP)

Code CSB-YP657126DOA
MSDS
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Source Yeast
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Code CSB-EP657126DOA
MSDS
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Source E.coli
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Code CSB-EP657126DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657126DOA
MSDS
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Source Baculovirus
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Code CSB-MP657126DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SUP
Uniprot No.
Alternative Names
SUP; FLO10; At3g23130; K14B15.1; Transcriptional regulator SUPERMAN
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-204
Target Protein Sequence
MERSNSIELR NSFYGRARTS PWSYGDYDNC QQDHDYLLGF SWPPRSYTCS FCKREFRSAQ ALGGHMNVHR RDRARLRLQQ SPSSSSTPSP PYPNPNYSYS TMANSPPPHH SPLTLFPTLS PPSSPRYRAG LIRSLSPKSK HTPENACKTK KSSLLVEAGE ATRFTSKDAC KILRNDEIIS LELEIGLINE SEQDLDLELR LGFA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcriptional regulator considered as cadastral protein that acts indirectly to prevent the B class homeotic proteins APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl. Principal function is to balance cell proliferation in the third and fourth whorls of developing flowers thereby maintaining the boundary between stamens and carpels. May fulfill this role by repressing genes implicated in cell division. Plays equally a role in the determinacy of the floral meristem. Is also required for normal ovule development.
Gene References into Functions
  1. SUPERMAN gene is required for proper specification of the boundary between stamens in whorl 3 and carpels in whorl 4, as superman mutants exhibit supernumerary stamens but usually lack carpels; the extra stamens in superman mutants arise from cells in whorl 4, which change their fate from female to male, while floral stem cells proliferate longer, allowing for the production of additional stamens PMID: 28634297
  2. Sup role in resolving male/female gradients into sharp male and female identities (whorls, organs) and in enforcing flower homeostasis during evolution PMID: 27098089
  3. Arabidopsis superman regulate hormone signalling and mediate cell proliferation and differentiation. PMID: 20980362
Subcellular Location
Nucleus.
Tissue Specificity
Expressed early in the third whorl, and later becomes localized at the boundary between the stamen and carpel whorls. Also detected in developing ovule primordia.
Database Links

KEGG: ath:AT3G23130

STRING: 3702.AT3G23130.1

UniGene: At.53448

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